Thakuria, Dwipendra, Talukdar, Narayan C. , Goswami, Chandan, Hazarika, Samarendra, Kalita, Mohan C. and Bending, G. D. (Gary D.). (2009) Evaluation of rice–legume–rice cropping system on grain yield, nutrient uptake, nitrogen fixation, and chemical, physical, and biological properties of soil. Biology and Fertility of Soils , Vol.45 (No.3). ISSN 0178-2762

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Abstract

To achieve higher yields and better soil quality under rice–legume–rice (RLR) rotation in a rainfed production system, we formulated integrated nutrient management (INM) comprised of Azospirillum (Azo), Rhizobium (Rh), and phosphate-solubilizing bacteria (PSB) with phosphate rock (PR), compost, and muriate of potash (MOP). Performance of bacterial bioinoculants was evaluated by determining grain yield, nitrogenase activity, uptake and balance of N, P, and Zn, changes in water stability and distribution of soil aggregates, soil organic C and pH, fungal/bacterial biomass C ratio, casting activities of earthworms, and bacterial community composition using denaturing gradient gel electrophoresis (DGGE) fingerprinting. The performance comparison was made against the prevailing farmers’ nutrient management practices [N/P2O5/K2O at 40:20:20 kg ha−1 for rice and 20:30:20 kg ha−1 for legume as urea/single super-phosphate/MOP (urea/SSP/MOP)]. Cumulative grain yields of crops increased by 7–16% per RLR rotation and removal of N and P by six crops of 2 years rotation increased significantly (P < 0.05) in bacterial bioinoculants-based INM plots over that in compost alone or urea/SSP/MOP plots. Apparent loss of soil total N and P at 0–15 cm soil depth was minimum and apparent N gain at 15–30 cm depth was maximum in Azo/Rh plus PSB dual INM plots. Zinc uptake by rice crop and diethylenetriaminepentaacetate-extractable Zn content in soil increased significantly (P < 0.05) in bacterial bioinoculants-based INM plots compared to other nutrient management plots. Total organic C content in soil declined at 0–15 cm depth and increased at 15–30 cm depth in all nutrient management plots after a 2-year crop cycle; however, bacterial bioinoculants-based INM plots showed minimum loss and maximum gain of total organic C content in the corresponding soil depths. Water-stable aggregation and distribution of soil aggregates in 53–250- and 250–2,000 μm classes increased significantly (P < 0.05) in bacterial bioinoculants-based INM plots compared to other nutrient management plots. Fungal/bacterial biomass C ratio seems to be a more reliable indicator of C and N dynamics in acidic soils than total microbial biomass C. Compost alone or Azo/Rh plus PSB dual INM plots showed significantly (P < 0.05) higher numbers of earthworms’ casts compared to urea/SSP/MOP alone and bacterial bioinoculants with urea or SSP-applied plots. Hierarchical cluster analysis based on similarity matrix of DGGE profiles revealed changes in bacterial community composition in soils due to differences in nutrient management, and these changes were seen to occur according to the states of C and N dynamics in acidic soil under RLR rotation.

Item Type:	Journal Article
Subjects:	S Agriculture > SB Plant culture
Divisions:	Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Fertilizers -- Research
Journal or Publication Title:	Biology and Fertility of Soils
Publisher:	Springer
ISSN:	0178-2762
Date:	February 2009
Volume:	Vol.45
Number:	No.3
Identification Number:	10.1007/s00374-008-0320-4
Status:	Peer Reviewed
Access rights to Published version:	Restricted or Subscription Access
Funder:	India. Dept. of Biotechnology
References:	Aulakh MS, Khera TS, Doran JW, Singh K, Singh B (2000) Yields and nitrogen dynamics in a rice-wheat system using green manure and inorganic fertilizer. Soil Sci Soc Am J 64:1867-1876 Bååth E, Anderson TH (2003) Comparison of soil fungal/bacterial ratios in a pH gradient using physiological and PLFA-based techniques. Soil Biol Biochem 35:955-963 Bailey VL, Smith JL, Bolton H (2002) Fungal to bacterial ratios in soils investigated for enhanced C sequestration. Soil Biol Biochem 34:997-1007 Barraquio WL, Daro MLG., Tirol AC, Ladha JK, Watanabe I (1986) Laboratory acetylene reduction assay for field grown wetland rice plants. Plant Soil 90:359-372 Boro RC, Goswami C, Thakuria D, Modi MK, Talukdar NC (2004) Molecular and functional characteristics, growth promoting effect and persistence of selected parent isolates and streptomycin resistant derivatives of rice rhizobacteria. Ind J Exp Biol 42:1186-1194 Bossuyt H, Denef K, Six J, Frey SD, Merchx R, Paustian K (2001) Influence of microbial populations and residue quality on aggregate stability. Appl Soil Ecol 16:195-208 Bray RH, Kurtz LT (1945) Determination of total, organic and available forms of phosphorus in soils. Soil Sci 59:39-45 Bremner JM, Mulvaney CS (1982) Nitrogen–total. In: Page AL, Miller RH, Keeney DR (eds) Methods of Soil Analysis, Part 2- Chemical and Microbiological properties. 2nd Edition, Agron Monogr 9, ASA, SSSA, CSSA, Madison, WI, pp 595-623 Cáceres EAR (1982) Improved medium for isolation of Azospirillum species. Appl Environ Microbiol 44:990-998 Camberdella CA, Elliott ET (1992) Particulate organic matter changes across a grassland cultivation sequence. Soil Sci Soc Am J 56:777-783 Choudhury ATMA, Kennedy IR (2004) Prospects and potentials for systems of biological nitrogen fixation in sustainable rice production. Biol Fertil Soils 39:219-227 Dalal RC, Mayer DG (1986). Long-term trends in fertility of soils under continuous cultivation and cereal cropping in southern Queensland. II. Total organic carbon and its rate of loss from the soil profile. Aust J Soil Res 24:281-292 DeDutta SK (1989) Integrated nutrient management in relation to soil fertility in lowland rice708 based cropping systems. In: Rice Farming Systems: New Directions. Proceedings of an International Symposium 31 January–3 February 1987. International Rice Research Institute, Manila, Philippines, pp. 141-160 Dice LR (1945) Measures of the amount of ecological association between species. Ecology 26:297-302 Dobermann A, White PF (1999) Strategies for nutrient management in irrigated and rainfed lowland rice systems. Nutr Cycl Agroecosys 53:1-18 Edwards CA, Arancon NQ (2004) Interactions among organic matter, earthworms, and microorganisms in promoting plant growth. In: Magdoff F, Weil RR (eds) Soil Organic Matter in Sustainable Agriculture, CRC Press, UK, pp. 328-363 Fernández LA, Zalba P, Gómez MA, Sagardoy MA (2007) Phosphate-solubilization activity of bacterial strains in soil and their effect on soyabean growth under greenhouse conditions. Biol Fertil Soils 43:805-809 George T, Ladha JK, Garrity DP, Buresh RJ (1994) Legume as ‘nitrate catch’ crops during the dry to wet transition in lowland rice cropping sstems. Agron J 86:267-273 Hafeel K, Rate AW, Abbott L (2004) Calibration of the substrate induced respiration and selective inhibition techniques for fungal bacterial ratios in Western Australian soils. SuperSoil 2004:3rd Australian New Zealand Soils Conference, 5-9 December, University of Sydney, Australia, http://www.regional.org.au/au/asssi/, pp 1-6 Hazra GC, Mandal B, Mandal LN (1987) Distribution of Zinc fractions and their transformation in submerged rice soils. Plant Soil 104:175-181 Jenkinson DS, Powlson DS (1976) The effects of biocidal treatments on metabolism in soil. V. A method for measuring soil biomass. Soil Biol Biochem 8:209-213 Jeyabal A, Kuppuswamy G (2001) Recycling of organic wastes for the production of vermicompost and its response in rice-legume cropping system and soil fertility. Euro J Agron 15:153-170 Johri BN, Sharma A, Virdi JS (2003) Rhizobaterial diversity in India and its influence on soil and plant health. Adv Biochem Engin/Biotechnol 84:49-89 Ju XT, Kou CL, Christie P, Dou ZX, Zhang FS (2007) Changes in soil environment from excessive application of fertilizers and manures to two contrasting intensive cropping systems on the North China Plain. Environ Pollut 145:497-506 Khan A, Chandra D, Nanda P, Singh S, Ghorai A (2004) Integrated nutrient management for sustainable rice production. Arch Agron Soil Sci. 50:161-165 Khan SA, Mulvaney RL, Ellsworh TR, Boast CW (2007) The myth of nitrogen fertilization for soil carbon sequestration. J Environ Qual 36:1821-1832 Kucey RMN, Janzen HH, Leggett ME (1989) Microbially mediated increase in plant744 available phosphorus. Adv Agron 42:199-228 Ladha JK, Reddy PM (2003) Nitrogen fixation in rice systems: state of knowledge and future prospects. Plant Soil 252:151-167 Liang J, Karamanos RE (1993) DTPA-extractable Fe, Mn, Cu and Zn. In. Carter MR (ed) Soil Sampling and Methods of Analysis, Canadian Society of Soil Science, Lewis Publishers, Canada, pp. 87-90 Lucy M, Reed E, Glick B R (2004) Applications of free living plant growth-promoting rhizobacteria. Antonie van Leeuwenhoek 86:1-25 Manna MC, Swarup A, Wanjari RH, Singh YV, Ghosh PK, Singh KN, Tripathi AK, Saha MN (2005) Soil organic matter in a West Bengal inceptisol after 30 years of multiple cropping and fertilization. Soil Sci Soc Am J 70:121-129 Muyzer G, De Waal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction757 amplified genes coding for 16S rRNA. Appl Environ Microbiol 59:695-700 Naklang K, Whitbread A, Lefroy R, Blair G, Wonprasaid S, Konboon Y, Suriya-arunroj D (1999) The management of rice straw, fertilizers and leaf litter in rice cropping systems in Northeast Thailand. 1. Soil carbon dynamics. Plant Soil 209:21-28 Nelson DW, Sommers LE (1982) Total carbon, organic carbon and organic matter. In: Page AL, Miller RH, Keeney DR (eds.) Methods of Soil Analysis. Part 2- Chemical and Microbiological properties, 2nd Edition, Agron Monogr 9: 961-1010, ASA, SSSA, CSSA, Madison, WI , pp. 539-594 Olsen SR, Sommers LE (1982) Phosphorus. In. Page AL, Miller RH, Keeney DR (eds) Methods of Soil Analysis, Part 2- Chemical and Microbiological properties. 2nd edition, Agron Monogr 9, ASA, SSSA, CSSA, Madison, WI, pp 403-430 Palaniappan SP (1985) Cropping system in the tropics. Wiley Eastern Ltd., New Delhi, India, pp 215 Pampolino MF, Manguiat IJ, Ramanathan S, Gines HC, Tan PS, Chi TTN, Rajendran R, Buresh RJ (2007) Environmental impact and economic benefits of site-specific nutrient management (SSNM) in irrigated rice systems. Agr Syst 93:1-24 Prasad R, Nagarajan S (2004) Rice–wheat cropping system – Food security and sustainability. Curr Sci 87:1134-1135 Raj A (2002) Biofertilizers for micronutrients. Biofertilizers Newletter 10:8-10 Reddy PR, Raju AP (2006) Integrated nutrient management for rice. Proceedings of 18th World Congress of Soil Science, 166-20: 4.2A Soil Care and Quality Soil Management, July 9-15, Philadelphia, Pennsylvania, USA Regmi AP, Ladha JK, Pathak H, Pasuquin E, Bueno C, Dawe D, Hobbs PR, Joshy D, Maskey SL, Pandey SP (2002) Yield and soil fertility trends in a 20-year rice-rice-wheat experiment in Nepal. Soil Sci Soc Am J 66:857-867 Roger PA, Ladha JK (1992) Biological N2-fixation in wetland rice fields: Estimation and contribution to nitrogen balance. Plant Soil 141:41-55 Roper MM, Ladha JK (1995) Biological N2 fixation by heterotrophic and phototrophic bacteria in association with straw. Plant Soil 174:211-224 Rosielle AA, Frey KJ (1975) Estimates of selection parameters associated with harvest index in oat lines derived from a bulk population. Euphytica 24:121-131 Somado EA, Becker M, Kuehne RF, Sahrawat KL, Vlek PLG (2003) Combined effects of legumes with rock phosphorus on rice in West Africa. Agron J 95:1172-1178 Srivastava SC, Singh JS (1989) Effect of cultivation on microbial carbon and nitrogen in dry tropical forest soil. Biol Fertil Soils 8:343-348 SubbaRao NS (1999) Soil Microbiology. 4th edition of Soil Microorganisms and Plant growth, Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi Sundara Rao WVB, Sinha MK (1963) Phosphate dissolving organisms in the soil and rhizosphere. Ind J Agric Sci 33:272-278 Talukdar NC, Bhattacharyya D, Hazarika S (2004) Soils and agriculture of Brahmaputra Basin. In: Singh VP, Sharma N (eds) Brahmaputra Basin Water Resources. Kluwer Academic Publishers, Holland, pp 94-112 Talukdar NC, Chakravarty DN (1988) Effects of varying levels of N, P and K on grain yield and nutrient uptake of Ahu rice in a light texture soil under high annual rainfall. Ann Agric Res 9:159-164 Tewari SN, Chakraborty DN, Bora PK (1969) Retention and transformation of soluble phosphopus added to Assam (India) soils. J Inst Chemists 61:174-176 Thakuria D (2006) Interactive effect of direct and indirect plant growth promoting bacteria on microbial community structure of rice rhizosphere. A Ph.D. Thesis. Department of Biotechnology, Gauhati University, Assam, India Thakuria D, Talukdar NC, Goswami C, Hazarika S, Boro RC, Khan MR (2004) Characterization and screening of bacteria from rhizosphere of rice grown in acidic soils of Assam. Curr Sci 86:978-985 Vessey JK (1994) Measurement of nitrogenase activity in legume root nodules: In defence of the acetylene reduction assay. Plant Soil 158:151-162 Wani SP, Chandrapalaih S, Zambre MA, Lee KK (1988) Association between N2 fixing bacteria and pearl millet plants: Responses, mechanisms and persistence. Plant Soil 110:289-302 Whalen JK, Parmelee RW, Edwards CA (1998) Population dynamics of earthworm communities in corn agroecosystems receiving organic or inorganic fertilizer amendments. Biol Fertil Soils 27:400-407 Witt C, Cassmann KG, Ottow JCG, Biker U (1998) Soil microbial biomass and nitrogen supply in an irrigated lowland rice soils as affected by crop rotation and residue management. Biol Fertil Soils 28:71-80 Witt C, Gaunt JL, Galicia CC, Ottow JCG, Neue HU (2000) A rapid chloroform fumigation extraction method for measuring soil microbial biomass carbon and N in flooded rice soils. Biol Fertil Soils 30:510-519 Yadav JSP (2003) Managing soil health for sustained high productivity. J Ind Soc Soil Sci 51:448-465 Yadvinder-Singh, Bijay-Singh, Ladha JK, Khind CS, Gupta RK, Meelu OP, Pasuquin E (2004) Long-term effects of organic inputs on yield and soil fertility in the rice-wheat rotation. Soil Sci Soc Am J 68:845-853 Yan D, Wang D, Yang L (2007) Long-tern effect of chemical fertilizer, straw, and manure on labile organic matter fractions in a paddy soil. Biol Fertil Soils 44:93-101 Zhang HC, Cao ZH, Shen QR, Wong MH (2003) Effect of phosphate fertilizer application on phosphorous (P) losses from paddy soils in Taihu Lake Region. I. Effect of phosphate fertilizer rate on P losses from paddy soil. Chemosphere 50:695-701
URI:	http://wrap.warwick.ac.uk/id/eprint/448

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