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Amoxicillin therapy of poultry flocks : effect upon the selection of amoxicillin-resistant commensal Campylobacter spp.
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Elviss, N. C., Williams, L. K., Jorgensen, F., Chisholm, S. A., Lawson, A. J., Swift, C., Owen, R. J., Griggs, Deborah, Johnson, M. M., Humphrey, T. J. and Piddock, Laura J. V. (2009) Amoxicillin therapy of poultry flocks : effect upon the selection of amoxicillin-resistant commensal Campylobacter spp. Journal of Antimicrobial Chemotherapy, Vol.64 (No.4). pp. 702-711. doi:10.1093/jac/dkp277 ISSN 0305-7453.
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Official URL: http://dx.doi.org/10.1093/jac/dkp277
Abstract
Background The aim of this study was to investigate the effect of amoxicillin therapy of poultry flocks upon the persistence of commensal Campylobacter spp. and the incidence of antibiotic resistance.
Methods Four poultry flocks naturally colonized with Campylobacter were treated with amoxicillin and monitored before, during and up to 4 weeks post-treatment. The numbers of Campylobacter were determined and the isolates speciated and typed by flaA short variable region (SVR) sequence analysis and PFGE. The susceptibility of the isolates to antibiotics, presence of the Cj0299 gene encoding a β-lactamase and β-lactamase production (nitrocefin hydrolysis) were also determined.
Results Amoxicillin-resistant Campylobacter were isolated from Flock 1 before and during treatment, but Campylobacter were not detected afterwards. Flock 2 was colonized by amoxicillin-susceptible strains throughout sampling. No amoxicillin-resistant isolates arose during or after treatment. Flock 3 contained amoxicillin-susceptible and -resistant types pre-treatment. Resistant isolates were detected during treatment, while antibiotic-susceptible isolates re-emerged at 3 weeks post-treatment. All Campylobacter isolates from Flock 4 were amoxicillin resistant, irrespective of sampling time. All but one of the 82 amoxicillin-resistant (MICs 16 to >128 mg/L) Campylobacter jejuni and Campylobacter coli tested for the presence of Cj0299 carried the gene and all of these produced β-lactamase. Co-amoxiclav remained active against amoxicillin-resistant isolates.
Conclusions Amoxicillin therapy had little effect on the numbers of amoxicillin-resistant commensal Campylobacter except for one flock where amoxicillin-resistant Campylobacter temporarily dominated. Amoxicillin therapy did not select amoxicillin-resistant isolates from a previous susceptible strain. Co-amoxiclav remained active against amoxicillin-resistant isolates.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | R Medicine > R Medicine (General) | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||
| Journal or Publication Title: | Journal of Antimicrobial Chemotherapy | ||||
| Publisher: | Oxford University Press | ||||
| ISSN: | 0305-7453 | ||||
| Official Date: | October 2009 | ||||
| Dates: |
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| Volume: | Vol.64 | ||||
| Number: | No.4 | ||||
| Page Range: | pp. 702-711 | ||||
| DOI: | 10.1093/jac/dkp277 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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