Weynberg, Karen Dawn, Allen, Michael J., Gilg, Ilana C., Scanlan, David J. and Wilson, William H.. (2011) Genome sequence of Ostreococcus tauri virus OtV-2 enlightens the role of picoeukaryote niche separation in the ocean. Journal of Virology, Vol.85 (No.9). pp. 4520-4529. ISSN 1098-5514

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Abstract

Ostreococcus tauri, a unicellular marine green alga, is the smallest known free-living eukaryote and is ubiquitous in the surface oceans. The ecological success of this organism has been attributed to distinct low- and high-light adapted ecotypes existing in different niches at a range of depths in the ocean. Viruses have already been characterised that infect the high-light adapted strains. Ostreococcus tauri virus isolate OtV-2 is a large double stranded DNA algal virus that infects a low-light adapted strain of O. tauri and was assigned to the algal virus family Phycodnaviridae, genus Prasinovirus. Our working hypothesis for this study was that different viruses infecting high-light vs. low-light adapted O. tauri strains would provide clues to propagation strategies that would give them selective advantages within their particular light niche. Sequence analysis of the 184,409 base pair linear OtV-2 genome revealed a range of core functional genes exclusive to this low-light genotype and included a variety of unexpected genes, such as those encoding a RNA polymerase sigma factor, at least four DNA methyltransferases, a cytochrome b5 and a high affinity phosphate transporter. It is clear that OtV-2 has acquired a range of potentially functional genes from its host, other eukaryotes and even bacteria over evolutionary time. Such piecemeal accretion of genes is a trademark of large doublestranded DNA viruses that has allowed them to adapt their propagation strategies to keep up with host niche separation in the sunlit layers of the oceanic environment.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Green algae -- Viruses -- Genetics
Journal or Publication Title:	Journal of Virology
Publisher:	American Society for Microbiology
ISSN:	1098-5514
Date:	May 2011
Volume:	Vol.85
Number:	No.9
Page Range:	pp. 4520-4529
Identification Number:	10.1128/JVI.02131-10
Status:	Peer Reviewed
Access rights to Published version:	Restricted or Subscription Access
Funder:	Natural Environment Research Council (Great Britain) (NERC), National Science Foundation (U.S.) (NSF)
Grant number:	NER/S/A/2005/13204, MGF196 (NERC) ; EF0949162 ( National Science Foundation)
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URI:	http://wrap.warwick.ac.uk/id/eprint/4546

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