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Multicopy proC in Streptomyces coelicolor A3(2) elicits a transient production of prodiginines, while proC deletion does not yield a proline auxotroph
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Barona-Gomez, Francisco and Hodgson, D. A. (2010) Multicopy proC in Streptomyces coelicolor A3(2) elicits a transient production of prodiginines, while proC deletion does not yield a proline auxotroph. Journal of Molecular Microbiology and Biotechnology, Volume 19 (Number 3). pp. 152-158. doi:10.1159/000321502 ISSN 1464-1801.
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Official URL: http://dx.doi.org/10.1159/000321502
Abstract
The last step of proline biosynthesis is typically catalysed by the enzyme Delta(1)-pyrroline-5-carboxylate reductase, encoded by the proC gene. Complete genome sequencing of Streptomyces coelicolor, a soil-dwelling Gram-positive bacterium that uses proline as a precursor for synthesis of prodiginine, revealed a single copy of this gene. Unexpectedly, disruption of this proC homologue (Sco3337) in S. coelicolor M145 yielded a prototrophic strain, yet the reductase activity of Sco3337 was confirmed by complementation of an Escherichia coli proC mutant. Multicopy proC within different genetic contexts elicited a transient production of prodiginines, which showed differential production kinetics of the two most common forms of this natural product produced by S. coelicolor, i.e. streptorubin B (cyclic) and undecylprodigiosin (linear). The metabolic and evolutionary implications of these observations are discussed. Copyright (C) 2010 S. Karger AG, Basel
Item Type: | Journal Article | ||||
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Subjects: | T Technology > TP Chemical technology Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Streptomyces coelicolor, Proline -- Synthesis, Protein C gene | ||||
Journal or Publication Title: | Journal of Molecular Microbiology and Biotechnology | ||||
Publisher: | S. Karger AG | ||||
ISSN: | 1464-1801 | ||||
Official Date: | 2010 | ||||
Dates: |
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Volume: | Volume 19 | ||||
Number: | Number 3 | ||||
Number of Pages: | 7 | ||||
Page Range: | pp. 152-158 | ||||
DOI: | 10.1159/000321502 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Funder: | Consejo Nacional de Ciencia y TecnologÃa (Mexico) [Mexican Council for Science and Technology] (CONACYT) | ||||
Grant number: | 111558, 82319 |
Data sourced from Thomson Reuters' Web of Knowledge
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