Barona-Gomez, Francisco and Hodgson, D. A. (David A.). (2010) Multicopy proC in Streptomyces coelicolor A3(2) elicits a transient production of prodiginines, while proC deletion does not yield a proline auxotroph. Journal of Molecular Microbiology and Biotechnology, Vol.19 (No.3). pp. 152-158. ISSN 1464-1801

Full text not available from this repository.

Abstract

The last step of proline biosynthesis is typically catalysed by the enzyme Delta(1)-pyrroline-5-carboxylate reductase, encoded by the proC gene. Complete genome sequencing of Streptomyces coelicolor, a soil-dwelling Gram-positive bacterium that uses proline as a precursor for synthesis of prodiginine, revealed a single copy of this gene. Unexpectedly, disruption of this proC homologue (Sco3337) in S. coelicolor M145 yielded a prototrophic strain, yet the reductase activity of Sco3337 was confirmed by complementation of an Escherichia coli proC mutant. Multicopy proC within different genetic contexts elicited a transient production of prodiginines, which showed differential production kinetics of the two most common forms of this natural product produced by S. coelicolor, i.e. streptorubin B (cyclic) and undecylprodigiosin (linear). The metabolic and evolutionary implications of these observations are discussed. Copyright (C) 2010 S. Karger AG, Basel

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology Q Science > QR Microbiology
Divisions:	Faculty of Science > Life Sciences (2010- )
Journal or Publication Title:	Journal of Molecular Microbiology and Biotechnology
Publisher:	S. Karger AG
ISSN:	1464-1801
Date:	2010
Volume:	Vol.19
Number:	No.3
Number of Pages:	7
Page Range:	pp. 152-158
Identification Number:	10.1159/000321502
Status:	Peer Reviewed
Publication Status:	Published
Funder:	Conacyt, Mexico
Grant number:	111558, 82319
URI:	http://wrap.warwick.ac.uk/id/eprint/4558

Data sourced from Thomson Reuters' Web of Knowledge

Request changes to a record

Actions (login required)

View Item