Alexandersson, Erik, Becker, John V. W., Jacobson, Dan, Nguema-Ona, Eric, Steyn, Cobus, Denby, Katherine J. and Vivier, Melané A.. (2011) Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco. BMC Research Notes, Vol.4 (No.1). p. 493. ISSN 1756-0500

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Abstract

Background Polygalacturonase-inhibiting proteins (PGIPs) directly limit the effective ingress of fungal pathogens by inhibiting cell wall-degrading endopolygalacturonases (ePGs). Transgenic tobacco plants over-expressing grapevine (Vitis vinifera) Vvpgip1 have previously been shown to be resistant to Botrytis infection. In this study we characterized two of these PGIP over-expressing lines with known resistance phenotypes by gene expression and hormone profiling in the absence of pathogen infection. Results Global gene expression was performed by a cross-species microarray approach using a potato cDNA microarray. The degree of potential cross-hybridization between probes was modeled by a novel computational workflow designed in-house. Probe annotations were updated by predicting probe-to-transcript hybridizations and combining information derived from other plant species. Comparing uninfected Vvpgip1-overexpressing lines to wild-type (WT), 318 probes showed significant change in expression. Functional groups of genes involved in metabolism and associated to the cell wall were identified and consequent cell wall analysis revealed increased lignin-levels in the transgenic lines, but no major differences in cell wall-derived polysaccharides. GO enrichment analysis also identified genes responsive to auxin, which was supported by elevated indole-acetic acid (IAA) levels in the transgenic lines. Finally, a down-regulation of xyloglucan endotransglycosylase/hydrolases (XTHs), which are important in cell wall remodeling, was linked to a decrease in total XTH activity. Conclusions This evaluation of PGIP over-expressing plants performed under pathogen-free conditions to exclude the classical PGIP-ePG inhibition interaction indicates additional roles for PGIPs beyond the inhibition of ePGs.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany S Agriculture > SB Plant culture
Divisions:	Faculty of Science > Life Sciences (2010- ) Faculty of Science > Centre for Systems Biology
Library of Congress Subject Headings (LCSH):	Polygalacturonase, Tobacco -- Genetics, Gene expression, Fungal diseases of plants
Journal or Publication Title:	BMC Research Notes
Publisher:	Bio Med Central
ISSN:	1756-0500
Date:	2011
Volume:	Vol.4
Number:	No.1
Page Range:	p. 493
Identification Number:	10.1186/1756-0500-4-493
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	National Research Foundation (South Africa), Wine Industry Network of Expertise and Technology, South African Table Grape Industry (SATI), South African Technology and Human Resources for Industry Programme (THRIP), Carl Tryggers Stiftelse för Vetenskaplig Forskning (Sweden), Claude Leon Foundation (South Africa)
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URI:	http://wrap.warwick.ac.uk/id/eprint/45609

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