The Library
High-resolution mapping of points of site-specific replication stalling
Tools
Vengrova, Sonya and Dalgaard, Jacob Z. (2009) High-resolution mapping of points of site-specific replication stalling. In: Vengrova, Sonya and Dalgaard, Jacob Z., (eds.) DNA replication : methods and protocols. Methods in molecular biology, Vol.521 . New York: Humana Press, pp. 215-227. ISBN 9781603278140
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1007/978-1-60327-815-7_12
Abstract
Genetic instability due to stalled replication forks is thought to underlie a number of human diseases, such as premature ageing and cancer susceptibility syndromes. In addition, site-specific stalling occurs at some genetic loci. A detailed understanding of the topology of the stalled replication fork gives a valuable insight into the causes and mechanisms of replication stalling. The method described here allows mapping of the position of the 3′-end of the nascent leading or lagging strand at the replication fork, stalled at a site-specific barrier. The replicating DNA is purified, digested with restriction enzymes, and enriched by BND-cellulose chromatography. The DNA is separated on a sequencing gel, transferred to a membrane, and hybridised to a strand-specific probe. The data obtained using this method allow determining the position of the 3′-end of the nascent strand at a stalled fork with a one-nucleotide resolution.
Item Type: | Book Item | ||||
---|---|---|---|---|---|
Subjects: | R Medicine > R Medicine (General) | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||
Series Name: | Methods in molecular biology | ||||
Journal or Publication Title: | Methods in Molecular Biology (Clifton, N.J.) | ||||
Publisher: | Humana Press | ||||
Place of Publication: | New York | ||||
ISBN: | 9781603278140 | ||||
ISSN: | 1064-3745 | ||||
Book Title: | DNA replication : methods and protocols | ||||
Editor: | Vengrova, Sonya and Dalgaard, Jacob Z. | ||||
Official Date: | 23 January 2009 | ||||
Dates: |
|
||||
Volume: | Vol.521 | ||||
Number of Pages: | 0 | ||||
Page Range: | pp. 215-227 | ||||
DOI: | 10.1007/978-1-60327-815-7_12 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |