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Human chorionic gonadotropin confers resistance to oxidative stress–induced apoptosis in decidualizing human endometrial stromal cells
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Kajihara, Takeshi, Uchino, Satomi, Suzuki, Motoharu, Itakura, Atsuo, Brosens, Jan J. and Ishihara, Osamu. (2011) Human chorionic gonadotropin confers resistance to oxidative stress–induced apoptosis in decidualizing human endometrial stromal cells. Fertility and Sterility, Vol.95 (No.4). pp. 1302-1307. ISSN 0015-0282
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Official URL: http://dx.doi.org/10.1016/j.fertnstert.2010.05.048
Abstract
Objective To investigate the effect of hCG on the expression of genes involved in oxidative stress resistance observed in decidualizing human endometrial stromal cells (HESCs). Design In vitro experiment. Setting Saitama Medical University hospital. Patient(s) Premenopausal women undergoing hysterectomy for myoma uteri. Intervention(s) HESCs from hysterectomy specimens were isolated and incubated with 8-bromo–cyclic adenosine monophosphate and medroxyprogesterone acetate in the presence or absence of recombinant hCG (rhCG) at various concentrations. Hydrogen peroxide was used as the source of reactive oxygen species (ROS). Main Outcome Measure(s) Apoptotic cells, FOXO1, superoxide dismutase 2 (SOD2), Bax, and Bcl-2 protein expression. Result(s) In a dose-dependent manner, rhCG conferred additional protection to decidualizing HESCs against oxidative stress–induced apoptosis. In parallel, rhCG augmented the expression of the forkhead transcription factor FOXO1 and its downstream target, the ROS scavenger SOD2. rhCG also inhibited the expression of the proapoptotic Bax protein and up-regulated antiapoptotic Bcl-2 levels in decidualizing HESCs exposed to ROS. Conclusion(s) The data suggest that hCG might improve the uterine environment upon implantation by suppressing apoptotic responses in the maternal decidua under oxidative stress.
| Item Type: | Journal Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Warwick Medical School > Reproductive Health Faculty of Medicine > Warwick Medical School |
| Journal or Publication Title: | Fertility and Sterility |
| Publisher: | Elsevier Inc. |
| ISSN: | 0015-0282 |
| Date: | 15 March 2011 |
| Volume: | Vol.95 |
| Number: | No.4 |
| Page Range: | pp. 1302-1307 |
| Identification Number: | 10.1016/j.fertnstert.2010.05.048 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| URI: | http://wrap.warwick.ac.uk/id/eprint/45857 |
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