Purohit, A., Fusi, L., Brosens, Jan J., Woo, L. W. L., Potter, B. V. L. and Reed, M. J.. (2008) Inhibition of steroid sulphatase activity in endometriotic implants by 667 COUMATE : a potential new therapy. Human Reproduction, Vol.23 (No.2). pp. 290-297. ISSN 0268-1161

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Abstract

BACKGROUND Local biosynthesis of estrogens is thought to be important for the maintenance and growth of endometriotic implants. In addition to the formation of estrogen via the aromatase pathway, steroid sulphatase (STS), which is responsible for the hydrolysis of estrogen sulphates, may be an important source of estrogens in endometriosis. METHODS Eutopic and ectopic endometrial samples from 14 women with minimal or mild (MM) endometriosis and from 13 women with moderate to severe (MS) endometriosis were analysed for aromatase and STS activities. RESULTS Aromatase and STS activity were detected in all samples. STS enzyme activity in both eutopic and ectopic endometrium was considerably higher and less variable than aromatase activity. Moreover, STS, but not aromatase, activity in endometriotic implants correlated with the severity of the disease (mean ± SEM: 203 ± 38 nmol/4 h/g wet weight tissue in MM disease versus 423 ± 44 nmol/4 h/g wet weight tissue in MS endometriosis, P < 0.001). The STS inhibitor 667 COUMATE almost completely blocked STS activity (>99%) in both eutopic and ectopic tissues. CONCLUSIONS The high levels of STS activity detected in ectopic endometrium and the correlation with severity of disease suggest that STS inhibitors could be useful for the treatment of endometriosis.

Item Type:	Journal Article
Subjects:	Q Science > QP Physiology R Medicine > RG Gynecology and obstetrics
Divisions:	Faculty of Medicine > Warwick Medical School > Reproductive Health Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Endometriosis -- Treatment -- Research, Endometriosis -- Etiology -- Research, Sulfatases, Sulfatases -- Inhibitors, Estrogen -- Synthesis
Journal or Publication Title:	Human Reproduction
Publisher:	OUP
ISSN:	0268-1161
Date:	February 2008
Volume:	Vol.23
Number:	No.2
Page Range:	pp. 290-297
Identification Number:	10.1093/humrep/dem308
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Sterix Ltd.
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URI:	http://wrap.warwick.ac.uk/id/eprint/45909

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