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Modulation of trophoblast angiogenic factor secretion by antiphospholipid antibodies is not reversed by heparin

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Carroll, Tamara Y., Mulla, Melissa J., Han, Christina S., Brosens, Jan J., Chamley, Larry W., Giles, Ian, Pericleous, Charis, Rahman, Anisur, Sfakianaki, Anna K., Paidas, Michael J. and Abrahams, Vikki M. (2011) Modulation of trophoblast angiogenic factor secretion by antiphospholipid antibodies is not reversed by heparin. American Journal of Reproductive Immunology, Vol.66 (No.4). pp. 286-296. doi:10.1111/j.1600-0897.2011.01007.x ISSN 1046-7408.

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Official URL: http://dx.doi.org/10.1111/j.1600-0897.2011.01007.x

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Abstract

Problem  Women with antiphospholipid antibodies (aPL) are at risk of miscarriage and pre-eclampsia, obstetrical disorders associated with reduced trophoblast invasion and spiral artery transformation. aPL target the placenta by binding beta2-glycoprotein I (β2GPI) on the trophoblast. In this study, we determined whether aPL alter the trophoblast secretion of angiogenic factors and evaluated the effect of low molecular weight heparin (LMWH) on this response.

Method of study  First-trimester trophoblast was treated with anti-β2GPI antibodies with or without LMWH. Angiogenic factor secretion was measured by enzyme-linked immunosorbent assay.

Results  Trophoblast cells produced more vascular endothelial growth factor (VEGF), placenta growth factor (PlGF), and soluble endoglin following exposure to anti-β2GPI Abs, and this occurred in both a MyD88-dependent and MyD88-independent manner. LMWH was unable to reverse the effects of the anti-β2GPI Abs on trophoblast VEGF secretion, but enhanced PlGF. Strikingly, LMWH upregulated soluble fms-like tyrosine kinase receptor-1 (sFlt-1) secretion independently of aPL.

Conclusion  This study demonstrates that aPL perturb the secretion of trophoblast angiogenic factors. LMWH does not reverse this effect but exacerbates sFlt-1 secretion, a potent anti-angiogenic factor. These findings may help to explain why women with antiphospholipid syndrome, who are treated with heparin to prevent early pregnancy loss, remain at increased risk of developing late obstetrical complications, such as pre-eclampsia.

Item Type: Journal Article
Subjects: R Medicine > RG Gynecology and obstetrics
Divisions: Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Reproductive Health ( - until July 2016)
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Journal or Publication Title: American Journal of Reproductive Immunology
Publisher: Wiley-Blackwell Publishing, Inc.
ISSN: 1046-7408
Official Date: October 2011
Dates:
DateEvent
October 2011Published
Volume: Vol.66
Number: No.4
Page Range: pp. 286-296
DOI: 10.1111/j.1600-0897.2011.01007.x
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

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