The Library
Transfusion of blood stored for longer periods of time does not alter the reactive hyperemia index in healthy volunteers
Tools
Coppadoro, A., Berra, L., Yu, B., Lei, C., Spagnolli, E., Steinbicker, A. U., Bloch, K. D., Lin, T., Warren, H. S., Sammy, F. Y., Fernandez, Bernadette O., Feelisch, Martin, Dzik, Walter, Stowell, Christopher P. and Zapol, Warren M. (2012) Transfusion of blood stored for longer periods of time does not alter the reactive hyperemia index in healthy volunteers. Critical Care, Vol.16 (Supp.1). S160. ISSN 1466-609X.
|
Text
WRAP_Feelisch_cc11055.pdf - Published Version Download (6Mb) | Preview |
Official URL: http://ccforum.com/content/16/S1/P448
Abstract
Introduction
The purpose of this study is to investigate the effects of transfusing human packed red blood cells (PRBC) after prolonged storage, as compared to short storage. Retrospective data suggest that transfusion of PRBC stored for over 2 weeks is associated with increased mortality and morbidity. During storage, PRBC progressively release hemoglobin, which avidly binds nitric oxide (NO). We hypothesized that the NO-mediated hyperemic response following ischemia would be reduced after transfusion of PRBC stored for 40 days.
Methods
We conducted a cross-over randomized interventional study, enrolling 10 healthy adults. Nine volunteers completed the study; one volunteer could not complete the protocol because of anemia. Each volunteer received 1 unit of 40-day and 1 unit of 3-day stored autologous leukoreduced PRBC, on different study days according to a randomization scheme. Blood withdrawal and reactive hyperemia index (RHI) measurements were performed before and 10 minutes, 1 hour, 2 hours, and 4 hours after transfusion.
Results
The change of RHI after transfusion of 40-day stored PRBC did not differ as compared to 3-day stored PRBC (P = 0.67). Plasma hemoglobin and bilirubin levels were higher after transfusion of 40-day than after 3-day stored PRBC (P = 0.02 and 0.001, respectively). Plasma levels of potassium, LDH, haptoglobin, cytokines, as well as blood pressure, did not differ between the two transfusions and remained within the normal range. Plasma nitrite concentrations increased after transfusion of 40-day stored PRBC, but not after transfusion of 3-day stored PRBC (P = 0.01).
Conclusion
Transfusion of 1 unit of autologous PRBC stored for longer periods of time is associated with increased hemolysis, an unchanged RHI and increased levels of plasma nitrite in healthy volunteers.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QP Physiology R Medicine > RC Internal medicine |
||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||
Library of Congress Subject Headings (LCSH): | Blood -- Transfusion, Hemolysis and hemolysins | ||||
Journal or Publication Title: | Critical Care | ||||
Publisher: | Bio Med Central | ||||
ISSN: | 1466-609X | ||||
Official Date: | 20 March 2012 | ||||
Dates: |
|
||||
Volume: | Vol.16 | ||||
Number: | Supp.1 | ||||
Page Range: | S160 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Description: | Abstract of poster presentation at the 32nd International Symposium on Intensive Care and Emergency Medicine, Brussels, Belgium, 20-23 March 2012 |
||||
Date of first compliant deposit: | 1 August 2016 | ||||
Date of first compliant Open Access: | 1 August 2016 |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year