The bZIP transcription factor MeaB mediates nitrogen metabolite repression at specific loci
Wagner, D., Schmeinck, A., Mos, Magdalena, Morozov, I. Y., Caddick, M. X. and Tudzynski, B.. (2010) The bZIP transcription factor MeaB mediates nitrogen metabolite repression at specific loci. Eukaryotic Cell, Vol.9 (No.10). pp. 1588-1601. ISSN 1535-9778Full text not available from this repository.
Official URL: http://dx.doi.org/10.1128/EC.00146-10
In Fusarium fujikuroi, bikaverin (BIK) biosynthesis is subject to repression by nitrogen. Unlike most genes subject to nitrogen metabolite repression, it has been shown that transcription of bik biosynthetic genes is not AreA dependent. Searching for additional transcription factors that may be involved in nitrogen regulation, we cloned and characterized the orthologue of Aspergillus nidulans meaB, which encodes a bZIP transcription factor. Two transcripts are derived from F. fujikuroi meaB: the large transcript (meaBL) predominates under nitrogen-sufficient conditions and the smaller transcript (meaBS) under nitrogen limitation, in an AreA-dependent manner. MeaB is specifically translocated to the nucleus under nitrogen-sufficient conditions in both F. fujikuroi and A. nidulans. Deletion of meaB resulted in partial upregulation of several nitrogen-regulated genes, but only in the ΔmeaB ΔareA double mutant were the bikaverin genes significantly upregulated in the presence of glutamine. These data demonstrate that MeaB and AreA coordinately mediate nitrogen metabolite repression and, importantly, that independently of AreA, MeaB can mediate nitrogen metabolite repression at specific loci in F. fujikuroi.
|Item Type:||Journal Article|
|Subjects:||R Medicine > R Medicine (General)|
|Divisions:||Faculty of Medicine > Warwick Medical School > Biomedical Cell Biology
Faculty of Medicine > Warwick Medical School
|Journal or Publication Title:||Eukaryotic Cell|
|Publisher:||American Society for Microbiology|
|Official Date:||October 2010|
|Page Range:||pp. 1588-1601|
|Access rights to Published version:||Restricted or Subscription Access|
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