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The Poly(C)-binding protein-1 regulates expression of the androgen receptor
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Cloke, Brianna, Shah, Kunal, Kaneda, Hiroshi, Lavery, Stuart, Trew, Geoffrey, Fusi, Luca, Higham, Jenny, Dina, Roberto E., Ghaem-Maghami, Sadaf, Ellis, Patricia, Brosens, Jan J. and Christian, Mark (2010) The Poly(C)-binding protein-1 regulates expression of the androgen receptor. Endocrinology, Vol.151 (No.8). pp. 3954-3964. doi:10.1210//en.2009-1264 ISSN 0013-7227.
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Official URL: http://dx.doi.org/10.1210/en.2009-1264
Abstract
The androgen receptor (AR) is a ligand-dependent transcription factor, expressed in male and female reproductive organs, and essential for normal reproduction in both sexes. The levels of AR are tightly controlled in androgen-responsive cells in which it plays a central role in the regulation of target gene expression. The AR is abundantly expressed in human endometrial stromal cells (HESCs), but levels decline markedly after differentiation into decidual cells in vivo and in primary cultures. Decidualization profoundly down-regulated AR protein levels with no discernible effect on either AR mRNA or protein stability, suggesting that loss of the receptor was a consequence of translational inhibition. Here we show that HESCs express three RNA-binding proteins, Hu antigen R and the poly(C)-binding proteins PCBP1 and PCBP2, that reportedly target the 3′-untranslated region of AR transcripts. Only PCBP1 expression was enhanced in secretory endometrium in vivo and in decidualizing HESCs. Furthermore, knockdown of PCBP1 in decidualizing cells was sufficient to restore AR protein levels, indicating that loss of the AR protein is primarily the consequence of a translational block. PCBP1 also blocked AR translation in a cell-free system, although this did not require binding to the 3′-untranslated region of the receptor mRNA. Furthermore, knockdown of PCBP1 in the prostate cancer LNCaP cell line also increased AR protein. Therefore, PCBP1 plays a major role in the dynamic expression of AR in both male and female androgen-responsive cells.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | R Medicine > R Medicine (General) | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Reproductive Health ( - until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | Endocrinology | ||||
| Publisher: | Endocrine Society | ||||
| ISSN: | 0013-7227 | ||||
| Official Date: | August 2010 | ||||
| Dates: |
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| Volume: | Vol.151 | ||||
| Number: | No.8 | ||||
| Page Range: | pp. 3954-3964 | ||||
| DOI: | 10.1210//en.2009-1264 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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