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Ionotropic NMDA and P2X(1/5) receptors mediate synaptically induced Ca2+ signalling in cortical astrocytes
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Palygin, Oleg, Lalo, Ulyana, Verkhratsky, Alexei and Pankratov, Yuriy (2010) Ionotropic NMDA and P2X(1/5) receptors mediate synaptically induced Ca2+ signalling in cortical astrocytes. Cell Calcium, Volume 48 (Number 4). pp. 225-231. doi:10.1016/j.ceca.2010.09.004 ISSN 0143-4160.
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Official URL: http://dx.doi.org/10.1016/j.ceca.2010.09.004
Abstract
Local, global and propagating calcium (Ca2+) signals provide the substrate for glial excitability. Here we analyse Ca2+ permeability of NMDA and P2X(1/5) receptors expressed in cortical astrocytes and provide evidence that activation of these receptors trigger astroglial Ca2+ signals when stimulated by either endogenous agonists or by synaptic release of neurotransmitters. The Ca2+ permeability of the ionotropic receptors was determined by reversal potential shift analysis; the permeability ratio P-Ca/P-K was 3.1 for NMDA receptors and 2.2 for P2X(1/5) receptors. Selective stimulation of ionotropic receptors (with NMDA and alpha,beta-methyleneATP) in freshly isolated cortical astrocytes induced ion currents associated with transient increases in cytosolic Ca2+ concentration ([Ca2+](i)). Stimulation of neuronal afferents in cortical slices triggered glial synaptic currents and [Ca2+](i) responses, which were partially blocked by selective antagonists of NMDA (D-AP5 and UBP141) and P2X(1/5) (NF449) receptors. We conclude that ionotropic receptors contribute to astroglial Ca2+ signalling and may provide a specific mechanism for fast neuronal-glial signalling at the synaptic level. (C) 2010 Elsevier Ltd. All rights reserved.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
Library of Congress Subject Headings (LCSH): | Astrocytes, Methyl aspartate -- Receptors, Purines -- Receptors, Cellular signal transduction, Calcium -- Physiological effect, Neuroglia, Neural transmission | ||||
Journal or Publication Title: | Cell Calcium | ||||
Publisher: | Churchill Livingstone | ||||
ISSN: | 0143-4160 | ||||
Official Date: | October 2010 | ||||
Dates: |
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Volume: | Volume 48 | ||||
Number: | Number 4 | ||||
Number of Pages: | 7 | ||||
Page Range: | pp. 225-231 | ||||
DOI: | 10.1016/j.ceca.2010.09.004 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Grantová agentura České republiky [Grant Agency of the Czech Republic] (GACR) | ||||
Grant number: | BB/F021445 (BBSRC), 305/08/1381, 305/08/1384 (GACR) |
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