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Ionotropic NMDA and P2X(1/5) receptors mediate synaptically induced Ca2+ signalling in cortical astrocytes
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Palygin, Oleg, Lalo, Ulyana, Verkhratsky, Alexei and Pankratov, Yuriy. (2010) Ionotropic NMDA and P2X(1/5) receptors mediate synaptically induced Ca2+ signalling in cortical astrocytes. Cell Calcium, Vol.48 (No.4). pp. 225-231. ISSN 0143-4160
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Official URL: http://dx.doi.org/10.1016/j.ceca.2010.09.004
Abstract
Local, global and propagating calcium (Ca2+) signals provide the substrate for glial excitability. Here we analyse Ca2+ permeability of NMDA and P2X(1/5) receptors expressed in cortical astrocytes and provide evidence that activation of these receptors trigger astroglial Ca2+ signals when stimulated by either endogenous agonists or by synaptic release of neurotransmitters. The Ca2+ permeability of the ionotropic receptors was determined by reversal potential shift analysis; the permeability ratio P-Ca/P-K was 3.1 for NMDA receptors and 2.2 for P2X(1/5) receptors. Selective stimulation of ionotropic receptors (with NMDA and alpha,beta-methyleneATP) in freshly isolated cortical astrocytes induced ion currents associated with transient increases in cytosolic Ca2+ concentration ([Ca2+](i)). Stimulation of neuronal afferents in cortical slices triggered glial synaptic currents and [Ca2+](i) responses, which were partially blocked by selective antagonists of NMDA (D-AP5 and UBP141) and P2X(1/5) (NF449) receptors. We conclude that ionotropic receptors contribute to astroglial Ca2+ signalling and may provide a specific mechanism for fast neuronal-glial signalling at the synaptic level. (C) 2010 Elsevier Ltd. All rights reserved.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) |
| Journal or Publication Title: | Cell Calcium |
| Publisher: | Churchill Livingstone |
| ISSN: | 0143-4160 |
| Date: | October 2010 |
| Volume: | Vol.48 |
| Number: | No.4 |
| Number of Pages: | 7 |
| Page Range: | pp. 225-231 |
| Identification Number: | 10.1016/j.ceca.2010.09.004 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Grant Agency of the Czech Republic (GACR) |
| Grant number: | BB/F021445 (BBSRC), 305/08/1381, 305/08/1384 |
| URI: | http://wrap.warwick.ac.uk/id/eprint/4688 |
Data sourced from Thomson Reuters' Web of Knowledge
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