Ionotropic NMDA and P2X(1/5) receptors mediate synaptically induced Ca2+ signalling in cortical astrocytes
Palygin, Oleg, Lalo, Ulyana, Verkhratsky, Alexei and Pankratov, Yuriy. (2010) Ionotropic NMDA and P2X(1/5) receptors mediate synaptically induced Ca2+ signalling in cortical astrocytes. Cell Calcium, Volume 48 (Number 4). pp. 225-231. ISSN 0143-4160Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.ceca.2010.09.004
Local, global and propagating calcium (Ca2+) signals provide the substrate for glial excitability. Here we analyse Ca2+ permeability of NMDA and P2X(1/5) receptors expressed in cortical astrocytes and provide evidence that activation of these receptors trigger astroglial Ca2+ signals when stimulated by either endogenous agonists or by synaptic release of neurotransmitters. The Ca2+ permeability of the ionotropic receptors was determined by reversal potential shift analysis; the permeability ratio P-Ca/P-K was 3.1 for NMDA receptors and 2.2 for P2X(1/5) receptors. Selective stimulation of ionotropic receptors (with NMDA and alpha,beta-methyleneATP) in freshly isolated cortical astrocytes induced ion currents associated with transient increases in cytosolic Ca2+ concentration ([Ca2+](i)). Stimulation of neuronal afferents in cortical slices triggered glial synaptic currents and [Ca2+](i) responses, which were partially blocked by selective antagonists of NMDA (D-AP5 and UBP141) and P2X(1/5) (NF449) receptors. We conclude that ionotropic receptors contribute to astroglial Ca2+ signalling and may provide a specific mechanism for fast neuronal-glial signalling at the synaptic level. (C) 2010 Elsevier Ltd. All rights reserved.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology
Q Science > QP Physiology
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Library of Congress Subject Headings (LCSH):||Astrocytes, Methyl aspartate -- Receptors, Purines -- Receptors, Cellular signal transduction, Calcium -- Physiological effect, Neuroglia, Neural transmission|
|Journal or Publication Title:||Cell Calcium|
|Number of Pages:||7|
|Page Range:||pp. 225-231|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Grantová agentura České republiky [Grant Agency of the Czech Republic] (GACR)|
|Grant number:||BB/F021445 (BBSRC), 305/08/1381, 305/08/1384 (GACR)|
Actions (login required)