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Differentiating Panax ginseng and Panax quinquefolius through SDS-PAGE analysis
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Yap, Kevin Yi-Lwern and Yeh, Jianmin. (2012) Differentiating Panax ginseng and Panax quinquefolius through SDS-PAGE analysis. International Journal of Biomedical and Pharmaceutical Sciences, 6 (Special Issue 1). pp. 119-122. ISSN 1752-3788
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Official URL: http://www.globalsciencebooks.info/JournalsSup/12I...
Abstract
Compound K (CK) is one of the principal metabolites of ginseng in human body which has been reported to exert diverse pharmaceutical activities including anticarcinogenic and antitumor effects on different lineages of cancer cells. However, the effect and mechanism of CK on colon cancer cells are not fully understood. In the present study the screening process was conducted with 12 different ginsenosides and metabolites, which showed CK was the most potent growth inhibitory compound against HT-29 colon cancer cells. The IC50 value of CK was 12.7 μM at 72 h. Cellular responses and growth pattern was analyzed simultaneously after CK treatment by Real Time Cellular Analysis (RTCA) method. CK treatment at specific concentration and time-point represented characteristic cytostatic stage in growth profile of HT-29 cells, and flow cytometric analysis showed that CK induced G1 phase arrest in cell cycle distribution followed by apoptosis. The G1 phase arrest was accompanied by down-regulation of cyclin D3, CDK6, and up-regulation of p21WAF-1/CIP1, and apoptosis was evidenced by inactivation of p-Bcl-2 and p-Akt. These results demonstrated that CK caused growth inhibition of HT-29 cells by blocking cells in G1 phase and inducing apoptosis.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QK Botany R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Faculty of Science > WMG (Formerly the Warwick Manufacturing Group) |
| Journal or Publication Title: | International Journal of Biomedical and Pharmaceutical Sciences |
| Publisher: | Global Science Books Ltd. |
| ISSN: | 1752-3788 |
| Date: | January 2012 |
| Volume: | 6 |
| Number: | Special Issue 1 |
| Page Range: | pp. 119-122 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| URI: | http://wrap.warwick.ac.uk/id/eprint/46958 |
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