Mukherjee, Arindam, Bilton, Paul R., Mackay, Logan, Janoschka, Adam, Zhu, Haizhong, Rea, Dean, Langridge-Smith, Pat R. R., Campopiano, Dominic J., Teschner, Thomas, Trautwein, Alfred, 1940-, Schünemann, Volker and Sadler, P. J.. (2012) Ferric ion (hydr)oxo clusters in the “Venus flytrap” cleft of FbpA : Mössbauer, calorimetric and mass spectrometric studies. JBIC Journal of Biological Inorganic Chemistry, Vol.17 (No.4). pp. 573-588. ISSN 0949-8257

Text WRAP_Mukherjee_480_JBIC-11-05-00115_Script_Rev3.pdf - Accepted Version Restricted to Repository staff only until 1 April 2013. Download (1303Kb)

Abstract

Isothermal calorimetric studies of the binding of iron(III) citrate to ferric ion binding protein from Neisseria gonorrhoeae suggested the complexation of a tetranuclear iron(III) cluster as a single step binding event (apparent binding constant K appITC = 6.0(5) × 105 M−1). High-resolution Fourier transform ion cyclotron resonance mass spectrometric data supported the binding of a tetranuclear oxo(hydroxo) iron(III) cluster of formula [Fe4O2(OH)4(H2O)(cit)]+ in the interdomain binding cleft of FbpA. The mutant H9Y-nFbpA showed a twofold increase in the apparent binding constant [K appITC = 1.1(7) × 106 M−1] for the tetranuclear iron(III) cluster compared to the wild-type protein. Mössbauer spectra of Escherichia coli cells overexpressing FbpA and cultured in the presence of added 57Fe citrate were indicative of the presence of dinuclear and polynuclear clusters. FbpA therefore appears to have a strong affinity for iron clusters in iron-rich environments, a property which might endow the protein with new biological functions.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QP Physiology
Divisions:	Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH):	Carrier proteins, Mössbauer spectroscopy, Transferrin, Calorimetry, Metal clusters, Mass spectrometry, Iron ions
Journal or Publication Title:	JBIC Journal of Biological Inorganic Chemistry
Publisher:	Springer
ISSN:	0949-8257
Date:	April 2012
Volume:	Vol.17
Number:	No.4
Page Range:	pp. 573-588
Identification Number:	10.1007/s00775-012-0878-z
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	European Commission (EC), Engineering and Physical Sciences Research Council (EPSRC), European Regional Development Fund (ERDF), Advantage West Midlands (AWM), European Research Council (ERC)
Grant number:	247450 (ERC)
References:	[1] Sutak R, Lesuisse E, Tachezy J, Richardson DR (2008) Trends Microbiol 16:261-268. [2] Nyilasi I, Papp T, Takó M, Nagy E, Vágvölgyi C (2005) Acta Microbiol Immunol Hung 52:185-97. [3] Mietzner TA, Tencza SB, Adhikari P, Vaughan KG, Nowalk AJ (1998) Curr Top Microbiol Immunol 225:113-35. [4] Sun H, Li H, Sadler PJ (1999) Chem. Rev. 99:2817-2842. [5] Weaver KD, Gabricevic M, Anderson DS, Adhikari P, Mietzner, TA, Crumbliss, AL (2010) Biochem 49:6021-6032. [6] Guo M, Harvey I, Campopiano DJ, Sadler PJ (2006) Angew Chem Int Ed Engl 45:2758-2761. [7] Zhong W, Alexeev D, Harvey I, Guo M, Hunter DJ, Zhu H, Campopiano DJ, Sadler PJ (2004) Angew Chem Int Ed 43:5914-5918. [8] Alexeev D, Zhu H, Guo M, Zhong W, Hunter DJ, Yang W, Campopiano DJ, Sadler PJ (2003) Nat Struct Biol 10:297-302. [9] Zhu H, Alexeev D, Hunter DJ, Campopiano DJ, Sadler PJ (2003) Biochem J 376:35-41. [10] Shouldice SR, Skene RJ, Dougan DR, McRee DE, Tari LW, Schryvers AB (2003) Biochemistry 42:11908-11914. [11] Lippard SJ (2005) Phil Trans R Soc A 363:861-877. [12] Decker A, Clay MD, Solomon EI (2006) J Inorg Biochem 100:697-706. [13] Vignais PM, Billoud B (2007) Chem Rev 107:4206-72. [14] Liu J, Lou Y, Yokota H, Adams PD, Kim R, Kim SH (2005) J Biol Chem 280:15960-15966. [15] Lill R, Mühlenhoff U (2008) Annu Rev Biochem 77:669-700. [16] Fontecave M, Ollagnier-de-Choudens S (2008) Arch Biochem Biophys 474:226-237. [17] Kovaleva EG, Neibergall MB, Chakrabarty S, Lipscomb JD (2007) Acc Chem Res 40:475-483. [18] Dhungana S, Anderson DS, Mietzner TA, Crumbliss AL (2004) J Inorg Biochem 98:1975-1977. [19] Zhu H, Alexeev D, Hunter DJ, Campopiano DJ, Sadler PJ (2003) Biochem J 376:35-41. [20] McRee DE, Bruns CM, Williams PA, Mietzner TA, Nunn R Research Collaboratory for Structural Bioinformatics protein Data Bank (RCSB PDB). DOI:10.2210/pdb1d9y/pdb [21] Alexeev D, Zhu H, Guo M, Zhong W, Hunter DJB, Yang W, Campopiano DJ, Sadler PJ (2003) Nat Struct Biol 10:297-302. [22] Zhong W, Alexeev D, Harvey I, Guo M, Hunter DJB, Zhu H, Campopiano DJ, Sadler PJ (2004) Angew Chem Int Ed 43:5914-5918. [23] Nowalk AJ, Vaughan KG, Day BW, Tencza SB, Mietzner TA (1997) Biochemistry 36:13054-13059. [24] Bekker EG, Creagh AL, Sanaie N, Yumoto F, Lau GHY, Tanokura M, Haynes CA, Murphy MEP (2004) Biochemistry 43:9195-9203. [25] Shouldice SR, Skene RJ, Dougan DR, Snell G, McRee DE, Schryvers AB, Tari LW (2004) J Bacteriol 186:3903-3910. [26] Shouldice SR, Dougan DR, Williams PA, Skene RJ, Snell G, Scheibe D, Kirby S, Hosfield DJ, McRee DE, Schryvers AB, Tari LW (2003) J Biol Chem 278:41093-41098. [27] Shouldice SR, McRee DE, Dougan DR, Tari LW, Schryvers AB (2005) J Biol Chem 280:5820-5827. [28] Tom-Yew SAL, Cui DT, Bekker EG, Murphy MEP (2005) J Biol Chem 280: 9283-9290. [29] Tom-Yew SAL, Shilton BH, Bekker EG, Tocheva EI, Murphy MEP. Research Collaboratory for Structural Bioinformatics protein Data Bank (RCSB PDB). DOI:10.2210/pdb2owt/pdb. [30] Bruns CM, Anderson, DS, Vaughan KG, Williams PA, Nowalk AJ, McRee DE, Mietzner TA (2001) Biochemistry 40:15631-15637. [31] Bruns CM, Nowalk AJ, Arvai AS, McTigue MA, Vaughan KG, Mietzner TA, McRee DE (1997) Nat Struct Biol 4:919-924. [32] Shouldice SR, Dougan DR, Skene RJ, Tari LW, McRee DE, Yu R-H, Schryvers AB (2003) J Biol Chem 278:11513-11519. [33] Khan AG, Shouldice SR, Tari LW, Schryvers AB (2007) Biochem J 403:43-48. [34] Khan AG, Shouldice SR, Kirby SD, Yu RH, Tari LW, Schryvers AB (2007) Biochem J 404:217-225. [35] Benda R, Tse Sum Bui B, Schunemann V, Florentin D, Marquet A, Trautwein AX (2002) Biochemistry 41:15000-15006. [36] Matzapetakis M, Raptopoulou CP, Tsohos A, Papaefthymiou V, Moon A, Salifoglou A (1998) J Am Chem Soc 120:13266-13267. [37] Guo M, Harvey I, Yang W, Coghill L, Campopiano DJ, Parkinson JA, MacGillivray RT, Harris WR, Sadler PJ (2003) J Biol Chem 278:2490-2502. [38] Pace CN, Vajdos F, Fee L, Grimsley G, Gray T (1995) Protein Sci. 4:2411-2423. [39] Ferreirós C, Criado MT, Gómez JA (1999) Comparative Biochemistry and Physiology B. 123:1–7. [40] Dhungana S, Taboy CH, Anderson DS, Vaughan KG, Aisen P, Mietzner TA, Crumbliss AL (2003) Proc Natl Acad Sci USA 100:3659-3664. [41] Schünemann V, Winkler H (2000) Rep Prog Phys 63: 263-353. [42] V. Schünemann und H. Paulsen (2007) Mössbauer Spectroscopy, in Application of Physical Methods to Inorganic and Bioinorganic Chemistry, edited by Robert A. Scott and Charles M. Lukehart, John Wiley and Sons, Ltd., ISBN: 978-0-470-03217. [43] Fox B-G, Hendrich M-P, Surerus K-K, Andersson K-K, Froland W-A, Libscomb J-D, Münck E (1993) J. Am. Chem. Soc. 115:3688 – 3701. [44] Lynch J-B, Juarez-Garcia C, Münck E, Que L Jr (1989) J. Biol. Chem. 264:8091 – 8096. [45] Tse Sum Bui B, Benda R, Schünemann V, Florentin D, Trautwein AX, Marquet A (2003) Biochemistry 42:8791-8798. [46] Lin L-N, Mason AB, Woodworth RC, Brandts JF (1991) Biochemistry 30:11660-1 1669. [47] Gautier-Luneau I, Merle C, Phanon D, Lebrun C, Biaso F, Serratrice G, Pierre J-L (2005) Chem. Eur. J. 11:2207-2219. [48] Shweky I, Bino A, Goldberg DP, Lippard SJ (1994) Inorg. Chem. 33:5161-5162. [49] Liu J-Q, Wang Y-Y, Ma L-F, Zhang W-H, Zeng X-R, Zhong F, Shi Q-Z, Peng S-M (2008) Inorg.Chim.Acta 361:173-182.
URI:	http://wrap.warwick.ac.uk/id/eprint/47171

Request changes to a record

Actions (login required)

View Item