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Characterization of simple isomeric oligosaccharides and the rapid separation of glycan mixtures by ion mobility mass spectrometry
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Williams, Jonathan P., Grabenauer, Megan, Holland, Richard J., Carpenter, Catherine J., Wormald, Mark R., Giles, Kevin, Harvey, David J., Bateman, Robert H., Scrivens, James H. and Bowers, Michael T. (2010) Characterization of simple isomeric oligosaccharides and the rapid separation of glycan mixtures by ion mobility mass spectrometry. International Journal of Mass Spectrometry, Vol.298 (Vol. 1-3). pp. 119-127. doi:10.1016/j.ijms.2009.08.006 ISSN 1387-3806.
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Official URL: http://dx.doi.org/10.1016/j.ijms.2009.08.006
Abstract
Ion mobility techniques, using both traveling wave-based technology and standard drift tube methods, along with molecular modeling were used to examine the gas-phase conformational properties of a series of isomeric oligosaccharides and hydrazine-released N-linked glycans from various sources. Electrospray ionization was used to generate H+ and Na+ adducts of oligosaccharides as well as Na+ and H2PO4- adducts of released N-linked glycans. The ion mobility mass spectrometry techniques were used to separate the isomeric oligosaccharides and the glycan mixtures. Good agreement was obtained between the theoretical and measured collision cross-sections. Glycans common to each glycoprotein were observed to have the same arrival time distribution independent of their source. In some cases support for multiple isomers was observed which correlated well with evidence obtained, where possible, from other experimental techniques. The sensitivity of the traveling wave ion mobility spectrometry (TWIMS) technique, together with the rapid experimental timescale, reproducibility and high information content make this an attractive approach for the characterization of complex mixtures of glycans released from glycoproteins. Successful calibration of the TWIMS arrival times/cross-sections was demonstrated using data from the drift tube instrument. (C) 2009 Elsevier B.V. All rights reserved.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QC Physics | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | International Journal of Mass Spectrometry | ||||
Publisher: | Elsevier BV | ||||
ISSN: | 1387-3806 | ||||
Official Date: | 1 December 2010 | ||||
Dates: |
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Volume: | Vol.298 | ||||
Number: | Vol. 1-3 | ||||
Number of Pages: | 9 | ||||
Page Range: | pp. 119-127 | ||||
DOI: | 10.1016/j.ijms.2009.08.006 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Department of Food and Rural Affairs, UK, National Science Foundation, USA, Oxford Glycobiology Institute |
Data sourced from Thomson Reuters' Web of Knowledge
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