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Transmembrane domain length is responsible for the ability of a plant reticulon to shape endoplasmic reticulum tubules in vivo

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Tolley, Nicholas, Sparkes, Imogen, Craddock, Christian P., Eastmond, Peter J., Runions, John, Hawes, C. R. and Frigerio, Lorenzo. (2010) Transmembrane domain length is responsible for the ability of a plant reticulon to shape endoplasmic reticulum tubules in vivo. Plant Journal, Volume 64 (Number 3). pp. 411-418. ISSN 0960-7412

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Official URL: http://dx.doi.org/10.1111/j.1365-313X.2010.04337.x

Abstract

Reticulons are integral endoplasmic reticulum (ER) membrane proteins that have the ability to shape the ER into tubules. It has been hypothesized that their unusually long conserved hydrophobic regions cause reticulons to assume a wedge-like topology that induces membrane curvature. Here we provide proof of this hypothesis. When over-expressed, an Arabidopsis thaliana reticulon (RTNLB13) localized to, and induced constrictions in, cortical ER tubules. Ectopic expression of RTNLB13 was sufficient to induce ER tubulation in an Arabidopsis mutant (pah1 pah2) whose ER membrane is mostly present in a sheet-like form. By sequential shortening of the four transmembrane domains (TMDs) of RTNLB13, we show that the length of the transmembrane regions is directly correlated with the ability of RTNLB13 to induce membrane tubulation and to form low-mobility complexes within the ER membrane. We also show that full-length TMDs are necessary for the ability of RTNLB13 to reside in the ER membrane.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QK Botany
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Endoplasmic reticulum, Plant organelles, Golgi apparatus
Journal or Publication Title:	Plant Journal
Publisher:	Blackwell
ISSN:	0960-7412
Official Date:	November 2010
Volume:	Volume 64
Number:	Number 3
Number of Pages:	8
Page Range:	pp. 411-418
Identification Number:	10.1111/j.1365-313X.2010.04337.x
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), European Union (EU)
Grant number:	LSH-2002-1.2.5-2 (EU)
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URI:	http://wrap.warwick.ac.uk/id/eprint/4778