McDonald, Tom O., Martin, Philip, Patterson, Joseph P., Smith, Darren, Giardiello, Marco, Marcello, Marco, See, Violaine, O'Reilly, Rachel K., Owen, Andrew and Rannard, Steve. (2012) Multicomponent organic nanoparticles for fluorescence studies in biological systems. Advanced Functional Materials, Vol.22 (No.12). pp. 2469-2478. ISSN 1616-301X

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Abstract

The formation of dual-component organic nanoparticles by a modified emulsion-templated freeze-drying approach leads to aqueous nanosuspensions showing fluorescence (Förster) resonance energy transfer (FRET) from within a distribution of single nanoparticles. The combination of both FRET dyes within dual-component nanoparticles (<200 nm) allows the spatial and physical monitoring of the particles, as the FRET signal is lost on dissolution and breakdown of the nanoparticles. The monitoring of accumulation by Caco-2 cells and macrophages shows very limited internalization within the non-phagocytic cells. Conservation of FRET within the macrophages confirms extensive whole-particle internalization. The cellular permeability through Caco-2 monolayers is also assessed and movement of intact dual-component particles is observed, suggesting a mechanism for enhanced pharmacokinetics in vivo.

Item Type:	Journal Article
Divisions:	Faculty of Science > Chemistry
Journal or Publication Title:	Advanced Functional Materials
Publisher:	John Wiley & Sons Ltd.
ISSN:	1616-301X
Date:	2012
Volume:	Vol.22
Number:	No.12
Page Range:	pp. 2469-2478
Identification Number:	10.1002/adfm.201103059
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
URI:	http://wrap.warwick.ac.uk/id/eprint/47933

Data sourced from Thomson Reuters' Web of Knowledge

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