Qin, Cheng, Shi, Nongnong, Gu, Mei, Zhang, Hang, Li, Bin, Shen, Jiajia, Mohammed, Atef, Ryabov, Eugene , Li, Chunyang, Wang, Huizhong, Liu, Yule, Osman, Toba, Vatish, Manu and Hong, Yiguo . (2012) Involvement of RDR6 in short-range intercellular RNA silencing in Nicotiana benthamiana. Scientific Reports, Vol.2 (No.467). ISSN 2045-2322

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Abstract

In plants, non-cell autonomous RNA silencing spreads between cells and over long distances. Recent work has revealed insight on the genetic and molecular components essential for cell-to-cell movement of RNA silencing in Arabidopsis. Using a local RNA silencing assay, we report on a distinct mechanism that may govern the short-range (6–10 cell) trafficking of virus-induced RNA silencing from epidermal to neighbouring palisade and spongy parenchyma cells in Nicotiana benthamiana. This process involves a previously unrecognised function of the RNA-dependent RNA polymerase 6 (RDR6) gene. Our data suggest that plants may have evolved distinct genetic controls in intercellular RNA silencing among different types of cells.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Medicine > Warwick Medical School > Clinical Sciences Research Institute (CSRI) Faculty of Science > Life Sciences (2010- ) Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Plant gene silencing, Nicotiana benthamiana -- Genetics
Journal or Publication Title:	Scientific Reports
Publisher:	Nature Publishing Group
ISSN:	2045-2322
Date:	2012
Volume:	Vol.2
Number:	No.467
Identification Number:	10.1038/srep00467
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	Special Foundation for Hangzhou Talents, Science Foundation of the Key Laboratory of Hangzhou, Guo jia zi ran ke xue ji jin wei yuan hui (China) [National Natural Science Foundation of China] (NSFC), Egypt. Wizārat al-Taʻlīm al-ʻĀlī wa-al-Dawlah lil-Buḥūth al-ʻIlmī, Warwick Ventures, Hangzhou shi fan da xue
Grant number:	20090232T05 (SFKLH), 30770185 (NSFC), 30870180 (NSFC)
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URI:	http://wrap.warwick.ac.uk/id/eprint/48064

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