Barnett, James P., Scanlan, David J. and Blindauer, Claudia A.. (2012) Protein fractionation and detection for metalloproteomics : challenges and approaches. Analytical and Bioanalytical Chemistry, Vol.402 (No.10). pp. 3311-3322. ISSN 1618-2642

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Abstract

At least one third of all proteins are thought to require a metal ion co-factor for their function. Recognition of the importance of metals in biological systems and major advances in analytical instrumentation and technology have led to the emergence of the new research area of metalloproteomics in recent years. Despite this progress, the experimental determination of in-vivo metal cofactors has remained challenging, because this requires elucidation of protein interactions with non-covalently bound metal ions. This critical review highlights current methodological approaches, focusing, in particular, on issues relating to the fractionation and separation of the metalloproteome, including recent experience with metalloproteomics for marine cyanobacteria in our laboratory. Metalloproteomics promises to deliver novel insights into fundamental biological processes in the future, but it is clear that further methodological advances are necessary to exploit the full potential of this emerging research area.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry
Divisions:	Faculty of Science > Chemistry
Journal or Publication Title:	Analytical and Bioanalytical Chemistry
Publisher:	Springer
ISSN:	1618-2642
Date:	April 2012
Volume:	Vol.402
Number:	No.10
Page Range:	pp. 3311-3322
Identification Number:	10.1007/s00216-012-5743-y
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
URI:	http://wrap.warwick.ac.uk/id/eprint/48185

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