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Regulators of G protein signalling proteins in the human myometrium

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Ladds, Graham , Zervou, Sevasti I., Vatish, Manu, Thornton, Steve and Davey, John . (2009) Regulators of G protein signalling proteins in the human myometrium. European Journal of Pharmacology, Vol.610 (No.1-3). pp. 23-28. ISSN 0014-2999

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Official URL: http://dx.doi.org/10.1016/j.ejphar.2009.03.042

Abstract

The contractile state of the human myometrium is controlled by extracellular signals that promote relaxation or contraction. Many of these signals function through G proteincoupled receptors at the cell surface, stimulating heterotrimeric G proteins and leading to changes in the activity of effector proteins responsible for bringing about the response. G proteins can interact with multiple receptors and many different effectors and are key players in the response. Regulators of G protein signalling (RGS) proteins are GTPase activating proteins for heterotrimeric G proteins and help terminate the signal. Little is known about the function of RGS proteins in human myometrium and we have therefore analysed transcript levels for RGS proteins at various stages of pregnancy (non-pregnant, preterm, term non-labouring, term labouring). RGS2 and RGS5 were the most abundantly expressed isolates in each of the patient groups. The levels of RGS4 and RGS16 (and to a lesser extent RGS2 and RGS14) increased in term labouring samples relative to the other groups. Yeast two-hybrid analysis and co-immunoprecipitation in myometrial cells revealed that both RGS2 and RGS5 interact directly with the cytoplasmic tail of the oxytocin receptor, suggesting they might help regulate signalling through this receptor. Key words: G protein-coupled receptors; labour; myometrium; RGS proteins

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Medicine > Warwick Medical School > Biomedical Cell Biology Faculty of Medicine > Warwick Medical School > Clinical Sciences Research Institute (CSRI) Faculty of Medicine > Warwick Medical School > Reproductive Health Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	G proteins, Cell receptors, Myometrium
Journal or Publication Title:	European Journal of Pharmacology
Publisher:	Elsevier BV
ISSN:	0014-2999
Date:	21 May 2009
Volume:	Vol.610
Number:	No.1-3
Number of Pages:	6
Page Range:	pp. 23-28
Identification Number:	10.1016/j.ejphar.2009.03.042
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	WellBeing (Organization), University Hospitals Coventry and Warwickshire NHS Trust
Grant number:	2198
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URI:	http://wrap.warwick.ac.uk/id/eprint/486