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A combinatorial TIR1/AFB–Aux/IAA co-receptor system for differential sensing of auxin
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Calderón Villalobos, Luz Irina A, Lee, Sarah C., Oliveira, Cesar de, Ivetac, Anthony, Brandt, Wolfgang, Armitage, Lynne, Sheard, Laura B., Tan, Xu, Parry, Geraint, Mao, Haibin, Zheng, Ning, Napier, R. (Richard), Kepinski, Stefan and Estelle, Mark (2012) A combinatorial TIR1/AFB–Aux/IAA co-receptor system for differential sensing of auxin. Nature Chemical Biology, Volume 8 (Number 5). pp. 477-485. doi:10.1038/nchembio.926 ISSN 1552-4450.
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Official URL: http://dx.doi.org/10.1038/nchembio.926
Abstract
The plant hormone auxin regulates virtually every aspect of plant growth and development. Auxin acts by binding the F-box protein transport inhibitor response 1 (TIR1) and promotes the degradation of the AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) transcriptional repressors. Here we show that efficient auxin binding requires assembly of an auxin co-receptor complex consisting of TIR1 and an Aux/IAA protein. Heterologous experiments in yeast and quantitative IAA binding assays using purified proteins showed that different combinations of TIR1 and Aux/IAA proteins form co-receptor complexes with a wide range of auxin-binding affinities. Auxin affinity seems to be largely determined by the Aux/IAA. As there are 6 TIR1/AUXIN SIGNALING F-BOX proteins (AFBs) and 29 Aux/IAA proteins in Arabidopsis thaliana, combinatorial interactions may result in many co-receptors with distinct auxin-sensing properties. We also demonstrate that the AFB5–Aux/IAA co-receptor selectively binds the auxinic herbicide picloram. This co-receptor system broadens the effective concentration range of the hormone and may contribute to the complexity of auxin response.
| Item Type: | Journal Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QK Botany | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
| Library of Congress Subject Headings (LCSH): | Auxin, Protein binding | ||||||||
| Journal or Publication Title: | Nature Chemical Biology | ||||||||
| Publisher: | Nature Publishing Group | ||||||||
| ISSN: | 1552-4450 | ||||||||
| Official Date: | 1 April 2012 | ||||||||
| Dates: |
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| Volume: | Volume 8 | ||||||||
| Number: | Number 5 | ||||||||
| Page Range: | pp. 477-485 | ||||||||
| DOI: | 10.1038/nchembio.926 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||
| Date of first compliant deposit: | 22 December 2015 | ||||||||
| Date of first compliant Open Access: | 22 December 2015 |
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