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Photoactivatable-GFP-α-tubulin as a tool to study microtubule plus-end turnover in living human cells
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Samora, Catarina P. and McAinsh, Andrew D. (2011) Photoactivatable-GFP-α-tubulin as a tool to study microtubule plus-end turnover in living human cells. In: Straube, Anne, (ed.) Microtubule Dynamics. Methods in Molecular Biology, Vol.777 . Humana Press, pp. 223-233. ISBN 9781617792519
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Official URL: http://dx.doi.org/10.1007/978-1-61779-252-6_16
Abstract
The development of photactivatable (PA) variants of Green fluorescent protein (GFP) has allowed the dynamics of spatially restricted protein pools within living cells to be determined. Over the last 5 years, experiments utilizing PA-GFP fused to α-tubulin have provided important insights into the mechanisms that control microtubule dynamics in living cells. In this chapter, we describe the methodology required to generate stable cell lines expressing photoactivatable-GFP-α-tubulin and to derive quantitative measurements of tubulin turnover at microtubules plus-ends in living cells.
| Item Type: | Book Item |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Cell Biology Faculty of Medicine > Warwick Medical School |
| Series Name: | Methods in Molecular Biology |
| Publisher: | Humana Press |
| ISBN: | 9781617792519 |
| ISSN: | 1064-3745 |
| Book Title: | Microtubule Dynamics |
| Editor: | Straube, Anne |
| Date: | 2011 |
| Volume: | Vol.777 |
| Page Range: | pp. 223-233 |
| Identification Number: | 10.1007/978-1-61779-252-6_16 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| URI: | http://wrap.warwick.ac.uk/id/eprint/48886 |
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