Hydrodynamic analysis of human kinetochore complexes during mitosis
McClelland, Sarah E. and McAinsh, Andrew D. (2009) Hydrodynamic analysis of human kinetochore complexes during mitosis. In: McAinsh, Andrew D., (ed.) Mitosis. Methods in Molecular Biology, Vol.545 . Humana Press, pp. 81-98. ISBN 9781603279925Full text not available from this repository.
Official URL: http://dx.doi.org/10.1007/978-1-60327-993-2_5
Hydrodynamic analysis is a powerful tool to dissect the molecular architecture of macromolecular protein assemblies. These techniques have been successfully used in yeast systems but are also well suited to the analysis of protein complexes from human cells. Furthermore, the combination of hydrodynamic analysis with siRNA mediated protein depletion provides an excellent system to probe the composition of protein complexes isolated from human cells. In this chapter we describe the use of these approaches in the analysis of macromolecular protein complexes during mitosis in human cells, using the kinetochore as an example.
|Item Type:||Book Item|
|Subjects:||R Medicine > R Medicine (General)|
|Divisions:||Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology
Faculty of Medicine > Warwick Medical School
|Series Name:||Methods in Molecular Biology|
|Editor:||McAinsh, Andrew D.|
|Page Range:||pp. 81-98|
|Access rights to Published version:||Restricted or Subscription Access|
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