Ahearne, Mark, Wilson, Samantha L., Liu, Kuo-Kang, Rauz, Saaeha, El Haj, Alicia J. and Yang, Ying. (2010) Influence of cell and collagen concentration on the cell-matrix mechanical relationship in a corneal stroma wound healing model. Experimental Eye Research, Vol.91 (No.5). pp. 584-591. ISSN 0014-4835

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Abstract

The effect of different collagen and cell concentrations on the mechanical and remodeling behaviors of corneal stroma wound healing models consisting of collagen hydrogels seeded with human corneal fibroblasts during a 25 day culture period were examined. Human corneal fibroblasts were seeded at 1 x 10(5), 3 x 10(5) or 5 x 10(5) cells per hydrogel, and collagen concentrations of 2.5 mg/ml, 3.5 mg/ml or 4.5 mg/ml were examined. Two non-destructive techniques, spherical indentation and optical coherence tomography, were used to measure the elastic modulus and dimensional changes respectively at several time-points over the culture period. The elastic modulus of the hydrogels increased continuously over 25 days. Hydrogels with higher initial cell seeding densities and lower initial collagen concentrations were found to increase in elastic modulus faster and possessed a higher elastic modulus by the end of the culture period when compared to the other hydrogels. A mathematical equation was applied to accurately fit the change in elastic modulus over time. This study demonstrates a robust in vitro technique able to monitor the effect of different parameters on the cell-matrix mechanical relationship in a corneal stroma model during prolonged culture periods and enhances our understanding on corneal wound healing processes. (C) 2010 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology R Medicine > RE Ophthalmology T Technology > TA Engineering (General). Civil engineering (General)
Divisions:	Faculty of Science > Engineering
Library of Congress Subject Headings (LCSH):	Cornea -- Mechanical properties, Colloids -- Mechanical properties, Fibroblasts, Wound healing -- Mathematical models, Cell interaction, Collagen, Elasticity -- Measurement
Journal or Publication Title:	Experimental Eye Research
Publisher:	Academic Press
ISSN:	0014-4835
Official Date:	November 2010
Volume:	Vol.91
Number:	No.5
Number of Pages:	8
Page Range:	pp. 584-591
Identification Number:	10.1016/j.exer.2010.07.013
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Birmingham Eye Foundation (UK) (BEF)
Grant number:	BB/F002866/1 (BBSRC), 257549 (BEF)
URI:	http://wrap.warwick.ac.uk/id/eprint/4897

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