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Influence of cell and collagen concentration on the cell-matrix mechanical relationship in a corneal stroma wound healing model
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Ahearne, Mark, Wilson, Samantha L., Liu, Kuo-Kang, Rauz, Saaeha, El Haj, Alicia J. and Yang, Ying. (2010) Influence of cell and collagen concentration on the cell-matrix mechanical relationship in a corneal stroma wound healing model. Experimental Eye Research, Vol.91 (No.5). pp. 584-591. ISSN 0014-4835
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Official URL: http://dx.doi.org/10.1016/j.exer.2010.07.013
Abstract
The effect of different collagen and cell concentrations on the mechanical and remodeling behaviors of corneal stroma wound healing models consisting of collagen hydrogels seeded with human corneal fibroblasts during a 25 day culture period were examined. Human corneal fibroblasts were seeded at 1 x 10(5), 3 x 10(5) or 5 x 10(5) cells per hydrogel, and collagen concentrations of 2.5 mg/ml, 3.5 mg/ml or 4.5 mg/ml were examined. Two non-destructive techniques, spherical indentation and optical coherence tomography, were used to measure the elastic modulus and dimensional changes respectively at several time-points over the culture period. The elastic modulus of the hydrogels increased continuously over 25 days. Hydrogels with higher initial cell seeding densities and lower initial collagen concentrations were found to increase in elastic modulus faster and possessed a higher elastic modulus by the end of the culture period when compared to the other hydrogels. A mathematical equation was applied to accurately fit the change in elastic modulus over time. This study demonstrates a robust in vitro technique able to monitor the effect of different parameters on the cell-matrix mechanical relationship in a corneal stroma model during prolonged culture periods and enhances our understanding on corneal wound healing processes. (C) 2010 Elsevier Ltd. All rights reserved.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QH Natural history > QH301 Biology R Medicine > RE Ophthalmology T Technology > TA Engineering (General). Civil engineering (General) |
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| Divisions: | Faculty of Science > Engineering | ||||
| Library of Congress Subject Headings (LCSH): | Cornea -- Mechanical properties, Colloids -- Mechanical properties, Fibroblasts, Wound healing -- Mathematical models, Cell interaction, Collagen, Elasticity -- Measurement | ||||
| Journal or Publication Title: | Experimental Eye Research | ||||
| Publisher: | Academic Press | ||||
| ISSN: | 0014-4835 | ||||
| Official Date: | November 2010 | ||||
| Dates: |
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| Volume: | Vol.91 | ||||
| Number: | No.5 | ||||
| Number of Pages: | 8 | ||||
| Page Range: | pp. 584-591 | ||||
| Identification Number: | 10.1016/j.exer.2010.07.013 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Birmingham Eye Foundation (UK) (BEF) | ||||
| Grant number: | BB/F002866/1 (BBSRC), 257549 (BEF) | ||||
| URI: | http://wrap.warwick.ac.uk/id/eprint/4897 |
Data sourced from Thomson Reuters' Web of Knowledge
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