Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Influence of cell and collagen concentration on the cell-matrix mechanical relationship in a corneal stroma wound healing model

Tools
- Tools
+ Tools

Ahearne, Mark, Wilson, Samantha L., Liu, Kuo-Kang, Rauz, Saaeha, El Haj, Alicia J. and Yang, Ying (2010) Influence of cell and collagen concentration on the cell-matrix mechanical relationship in a corneal stroma wound healing model. Experimental Eye Research, Vol.91 (No.5). pp. 584-591. doi:10.1016/j.exer.2010.07.013

Research output not available from this repository.

Request-a-Copy directly from author or use local Library Get it For Me service.

Official URL: http://dx.doi.org/10.1016/j.exer.2010.07.013

Request Changes to record.

Abstract

The effect of different collagen and cell concentrations on the mechanical and remodeling behaviors of corneal stroma wound healing models consisting of collagen hydrogels seeded with human corneal fibroblasts during a 25 day culture period were examined. Human corneal fibroblasts were seeded at 1 x 10(5), 3 x 10(5) or 5 x 10(5) cells per hydrogel, and collagen concentrations of 2.5 mg/ml, 3.5 mg/ml or 4.5 mg/ml were examined. Two non-destructive techniques, spherical indentation and optical coherence tomography, were used to measure the elastic modulus and dimensional changes respectively at several time-points over the culture period. The elastic modulus of the hydrogels increased continuously over 25 days. Hydrogels with higher initial cell seeding densities and lower initial collagen concentrations were found to increase in elastic modulus faster and possessed a higher elastic modulus by the end of the culture period when compared to the other hydrogels. A mathematical equation was applied to accurately fit the change in elastic modulus over time. This study demonstrates a robust in vitro technique able to monitor the effect of different parameters on the cell-matrix mechanical relationship in a corneal stroma model during prolonged culture periods and enhances our understanding on corneal wound healing processes. (C) 2010 Elsevier Ltd. All rights reserved.

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH301 Biology
R Medicine > RE Ophthalmology
T Technology > TA Engineering (General). Civil engineering (General)
Divisions: Faculty of Science, Engineering and Medicine > Engineering > Engineering
Library of Congress Subject Headings (LCSH): Cornea -- Mechanical properties, Colloids -- Mechanical properties, Fibroblasts, Wound healing -- Mathematical models, Cell interaction, Collagen, Elasticity -- Measurement
Journal or Publication Title: Experimental Eye Research
Publisher: Academic Press
ISSN: 0014-4835
Official Date: November 2010
Dates:
DateEvent
November 2010Published
Volume: Vol.91
Number: No.5
Number of Pages: 8
Page Range: pp. 584-591
DOI: 10.1016/j.exer.2010.07.013
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Birmingham Eye Foundation (UK) (BEF)
Grant number: BB/F002866/1 (BBSRC), 257549 (BEF)

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us