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Phosphodependent recruitment of Bub1 and Bub3 to Spc7/KNL1 by Mph1 kinase maintains the spindle checkpoint

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Shepperd, Lindsey A., Meadows, John C., Sochaj, Alicja M., Lancaster, Theresa C., Zou, Juan, Buttrick, Graham J., Rappsilber, Juri, Hardwick, Kevin G. and Millar, Jonathan B. A. (2012) Phosphodependent recruitment of Bub1 and Bub3 to Spc7/KNL1 by Mph1 kinase maintains the spindle checkpoint. Current Biology, Vol.22 (No.10). pp. 891-899. doi:10.1016/j.cub.2012.03.051

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Official URL: http://dx.doi.org/10.1016/j.cub.2012.03.051

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Abstract

The spindle assembly checkpoint (SAC) is the major surveillance system that ensures that sister chromatids do not separate until all chromosomes are correctly bioriented during mitosis. Components of the checkpoint include Mad1, Mad2, Mad3 (BubR1), Bub3, and the kinases Bub1, Mph1 (Mps1), and Aurora B [1]. Checkpoint proteins are recruited to kinetochores when individual kinetochores are not bound to spindle microtubules or not under tension [2,3,4,5]. Kinetochore association of Mad2 causes it to undergo a conformational change, which promotes its association to Mad3 and Cdc20 to form the mitotic checkpoint complex (MCC). The MCC inhibits the anaphase-promoting complex/cyclosome (APC/C) until the checkpoint is satisfied. SAC silencing derepresses Cdc20-APC/C activity. This triggers the polyubiquitination of securin and cyclin, which promotes the dissolution of sister chromatid cohesion and mitotic progression [6,7,8]. We, and others, recently showed that association of PP1 to the Spc7/Spc105/KNL1 family of kinetochore proteins is necessary to stabilize microtubule-kinetochore attachments and silence the SAC [9,10,11,12]. We now report that phosphorylation of the conserved MELT motifs in Spc7 by Mph1 (Mps1) recruits Bub1 and Bub3 to the kinetochore and that this is required to maintain the SAC signal.

Item Type: Journal Article
Divisions: Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology
Faculty of Medicine > Warwick Medical School
Journal or Publication Title: Current Biology
Publisher: Cell Press
ISSN: 0960-9822
Official Date: 2012
Dates:
DateEvent
2012Published
Volume: Vol.22
Number: No.10
Page Range: pp. 891-899
DOI: 10.1016/j.cub.2012.03.051
Status: Peer Reviewed
Publication Status: Published

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