The Library

MYOD-1 in normal colonic mucosa : role as a putative biomarker?

Tools

Arasaradnam, Ramesh P., Quraishi, M. N., Commane, Daniel M., Mathers, John C. and Bradburn, Mike. (2012) MYOD-1 in normal colonic mucosa : role as a putative biomarker? BMC Research Notes, Vol.5 (No.1). p. 240. ISSN 1756-0500

[img]
Preview
 Text
WRAP_Arasaradnam_1756-0500-5-240.pdf - Published Version
Download (438Kb) | Preview

Abstract

Background
DNA methylation of promoter-associated CpG islands of certain genes may play a role in the development of colorectal cancer. The MYOD-1 gene which is a muscle differentiation gene has been showed to be significantly methylated in colorectal cancer which, is an age related event. However the role of this gene in the colonic mucosa is not understood and whether methylation occurs in subjects without colon cancer. In this study, we have determined the frequency of methylation of the MYOD-1 gene in normal colonic mucosa and investigated to see if this is associated with established colorectal cancer risk factors primarily ageing.

Results
We analysed colonic mucosal biopsies in 218 normal individuals and demonstrated that in most individuals promoter hypermethylation was not quantified for MYOD-1. However, promoter hypermethylation increased significantly with age (p < 0.001 using regression analysis) and this was gender independent. We also showed that gene promoter methylation increased positively with an increase in waist to hip (WHR) ratio – the latter is also a known risk factor for colon cancer development.

Conclusions
Our study suggests that promoter gene hypermethylation of the MYOD-1 gene increases significantly with age in normal individuals and thus may offer potential as a putative biomarker for colorectal cancer.

Item Type: Journal Article
Subjects: Q Science > QP Physiology
Divisions: Faculty of Medicine > Warwick Medical School > Clinical Sciences Research Institute (CSRI)
Library of Congress Subject Headings (LCSH): Colon (Anatomy) -- Cancer -- Genetic aspects, Rectum -- Cancer -- Genetic aspects, DNA -- Methylation, Colon (Anatomy) -- Physiology
Journal or Publication Title: BMC Research Notes
Publisher: Bio Med Central
ISSN: 1756-0500
Official Date: 2012
Volume: Vol.5
Number: No.1
Page Range: p. 240
Identification Number: 10.1186/1756-0500-5-240
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Great Britain. Food Standards Agency (FSA)
Grant number: N12105 (FSA)
References:

1. Kinzler KW, Vogelstein B: Lessons from hereditary colorectal cancer. Cell
1996, 87(2):159–170.
2. Jones PA, Baylin SB: The fundamental role of epigenetic events in cancer.
Nat Rev Genet 2002, 3(6):415–428.
3. Jones PA: DNA Methylation errors and cancer. Cancer Res 1996, 56
(11):2463–2467.
4. Agrawal A, Murphy RF, Agrawal DK: DNA methylation in breast and
colorectal cancers. Mod Pathol 2007, 20(7):711–721.
5. Issa JP, Ottaviano YL, Celano P, Hamilton SR, Davidson NE, Baylin SB:
Methylation of the oestrogen receptor CpG island links ageing and
neoplasia in human colon. Nat Genetics 1994, 7(4):536–540.
6. Arasaradnam RP, Commane DM, Bradburn D, Mathers JC: A review of
dietary factors and its influence on DNA methylation in colorectal
carcinogenesis. Epigenetics 2008, 3(4):193–198.
7. Wallner M, Herbst A, Behrens A, Crispin A, Stieber P, Göke B, et al:
Methylation of serum DNA is an independent prognostic marker in
colorectal cancer. Clin Cancer Res 2006, 12:7347–7352.
8. Ahuja N, Li Q, Mohan AL, Baylin SB, Issa JP: Aging and DNA methylation in
colorectal mucosa and cancer. Cancer Res 1998, 58(23):5489–5494.
9. Hiranuma C, Kawakami K, Oyama K, Ota N, Omura K, Watanabe G:
Hypermethylation of the MYOD1 gene is a novel prognostic factor in
patients with colorectal cancer. Int J Mol Med 2004, 13(3):413–417.
10. Megeney LA, Kablar B, Garrett K, Anderson JE, Rudnicki MA: MyoD is
required for myogenic stem cell function in adult skeletal muscle. Genes
Dev 1996, 10:1173–1183.
11. Davis RL, Cheng PF, Lassar AB, Weintraub H: The MyoD DNA binding
domain contains recognition code for muscle-specific gene activation.
Cell 1990, 60(5):733–746.
12. Cancer Research UK: Cancer Statistics Registrations: Registrations of cancer
diagnosed in 2007. England: Office for National Statistics. Available at: http://
www.ons.gov.uk/ons/rel/vsob1/cancer-statistics-registrations–england–seriesmb1-/
no–39–2008/cancer-statistics–registrations-series-mb1.pdf (Accessed:
2nd July 2011).
13. Ye C, Shrubsole MJ, Cai Q, Ness R, Grady WM, Smalley W: Promoter
methylation status of the MGMT, hMLH1, and CDKN2A/p16 genes in
non-neoplastic mucosa of patients with and without colorectal
adenomas. Oncol Rep 2006, 16(2):429–435.
14. Kawakami K, Ruszkiewicz A, Bennett G, Moore J, Grieu F, Watanabe G: DNA
hypermethylation in the normal colonic mucosa of patients with
colorectal cancer. Br J Cancer 2006, 94(4):593–598.
15. Issa JP, Ahuja N, Toyota M, Bronner MP, Brentnall TA: Accelerated Agerelated
CpG Island Methylation in Ulcerative Colitis. Cancer Res 2001, 61
(9):3573–3577.
16. Xu XL, Yu J, Zhang HY, Sun MH, Gu J, Du X, Shi DR: Methylation profile of
the promoter CpG islands of 31 genes that may contribute to colorectal
carcinogenesis. World J Gastroenterology 2004, 10(23):3441–3454.
17. Chen X, Liu H, Li L, Si J, Wang J: Expression of the MYOD Gene Family on
Small Intestine development of ducks. China: China Poultry; 2010:8.
18. Friedenreich C, Norat T, Steindorf K, Boutron-Ruault MC, Pischon T, Mazuir
M, et al: Physical activity and risk of colon and rectal cancers: the
European prospective investigation into cancer and nutrition. Cancer
Epidemiol Biomarkers Prev 2006, 15(12):2398–2407.
19. Xiong Z, Laird P: COBRA: a sensitive and quantitative DNA methylation
assay. Nucl. Acids Res. 1997, 25(12):2532–2534.
URI: http://wrap.warwick.ac.uk/id/eprint/49222

Request changes to a record

Actions (login required)

View Item View Item

Document Downloads

More statistics for this item...