MYOD-1 in normal colonic mucosa : role as a putative biomarker?
Arasaradnam, Ramesh P., Quraishi, M. N., Commane, Daniel M., Mathers, John C. and Bradburn, Mike. (2012) MYOD-1 in normal colonic mucosa : role as a putative biomarker? BMC Research Notes, Vol.5 (No.1). p. 240. ISSN 1756-0500
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Official URL: http://dx.doi.org/10.1186/1756-0500-5-240
DNA methylation of promoter-associated CpG islands of certain genes may play a role in the development of colorectal cancer. The MYOD-1 gene which is a muscle differentiation gene has been showed to be significantly methylated in colorectal cancer which, is an age related event. However the role of this gene in the colonic mucosa is not understood and whether methylation occurs in subjects without colon cancer. In this study, we have determined the frequency of methylation of the MYOD-1 gene in normal colonic mucosa and investigated to see if this is associated with established colorectal cancer risk factors primarily ageing.
We analysed colonic mucosal biopsies in 218 normal individuals and demonstrated that in most individuals promoter hypermethylation was not quantified for MYOD-1. However, promoter hypermethylation increased significantly with age (p < 0.001 using regression analysis) and this was gender independent. We also showed that gene promoter methylation increased positively with an increase in waist to hip (WHR) ratio – the latter is also a known risk factor for colon cancer development.
Our study suggests that promoter gene hypermethylation of the MYOD-1 gene increases significantly with age in normal individuals and thus may offer potential as a putative biomarker for colorectal cancer.
|Item Type:||Journal Article|
|Subjects:||Q Science > QP Physiology|
|Divisions:||Faculty of Medicine > Warwick Medical School > Clinical Sciences Research Institute (CSRI)|
|Library of Congress Subject Headings (LCSH):||Colon (Anatomy) -- Cancer -- Genetic aspects, Rectum -- Cancer -- Genetic aspects, DNA -- Methylation, Colon (Anatomy) -- Physiology|
|Journal or Publication Title:||BMC Research Notes|
|Publisher:||Bio Med Central|
|Page Range:||p. 240|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Great Britain. Food Standards Agency (FSA)|
|Grant number:||N12105 (FSA)|
1. Kinzler KW, Vogelstein B: Lessons from hereditary colorectal cancer. Cell
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