McDowell, A. (Andrew), Barnard, E. (Emma), Nagy, I. (István), Gao, Anna, Tomida, Shuta, Li, Huiying, Eady, Anne, Cove, Jonathan, Nord, Carl Erik and Patrick, Sheila. (2012) An expanded multilocus sequence typing scheme for propionibacterium acnes : investigation of 'pathogenic', 'commensal' and antibiotic resistant strains. PLoS ONE, Vol.7 (Vol.7). e41480. ISSN 1932-6203

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Abstract

The Gram-positive bacterium Propionibacterium acnes is a member of the normal human skin microbiota and is associated with various infections and clinical conditions. There is tentative evidence to suggest that certain lineages may be associated with disease and others with health. We recently described a multilocus sequence typing scheme (MLST) for P. acnes based on seven housekeeping genes (http://pubmlst.org/pacnes). We now describe an expanded eight gene version based on six housekeeping genes and two ‘putative virulence’ genes (eMLST) that provides improved high resolution typing (91eSTs from 285 isolates), and generates phylogenies congruent with those based on whole genome analysis. When compared with the nine gene MLST scheme developed at the University of Bath, UK, and utilised by researchers at Aarhus University, Denmark, the eMLST method offers greater resolution. Using the scheme, we examined 208 isolates from disparate clinical sources, and 77 isolates from healthy skin. Acne was predominately associated with type IA1 clonal complexes CC1, CC3 and CC4; with eST1 and eST3 lineages being highly represented. In contrast, type IA2 strains were recovered at a rate similar to type IB and II organisms. Ophthalmic infections were predominately associated with type IA1 and IA2 strains, while type IB and II were more frequently recovered from soft tissue and retrieved medical devices. Strains with rRNA mutations conferring resistance to antibiotics used in acne treatment were dominated by eST3, with some evidence for intercontinental spread. In contrast, despite its high association with acne, only a small number of resistant CC1 eSTs were identified. A number of eSTs were only recovered from healthy skin, particularly eSTs representing CC72 (type II) and CC77 (type III). Collectively our data lends support to the view that pathogenic versus truly commensal lineages of P. acnes may exist. This is likely to have important therapeutic and diagnostic implications.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Propionibacterium acnes -- Genetics
Journal or Publication Title:	PLoS ONE
Publisher:	PLOS
ISSN:	1932-6203
Date:	30 July 2012
Volume:	Vol.7
Number:	Vol.7
Page Range:	e41480
Identification Number:	10.1371/journal.pone.0041480
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	Northern Ireland. Health and Personal Social Services Executive, Prostate Cancer UK, Hungary. Nemzeti Kutatási és Technológiai Hivatal [National Office for Research and Technology], French-Hungarian Associated European Laboratory (LEA), Micropathology Ltd., Great Britain. Royal Commission for the Exhibition of 1851
Grant number:	9.41 (HSCNI), 110831 (PCUK), OMFB-00441/2007 (NKTH), OMFB-00272/2009 (LEA), TAMOP-4.2.1.B-10/2/KONV-2010-0002 (LEA)
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URI:	http://wrap.warwick.ac.uk/id/eprint/49237

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