The Library
Role of hyaluronic acid in maturation and further early embryo development of bovine oocytes
Tools
Tools
Tools
Marei, W. F., Ghafari, F. and Fouladi-Nashta, A. A. (2012) Role of hyaluronic acid in maturation and further early embryo development of bovine oocytes. Theriogenology, Vol.78 (No.3). pp. 670-677. doi:10.1016/j.theriogenology.2012.03.013
Full text not available from this repository, contact author.
Official URL: http://dx.doi.org/10.1016/j.theriogenology.2012.03...
Abstract
Hyaluronic acid (HA), an important component of the extracellular matrix, plays a crucial role for cumulus cell expansion. Genes and proteins involved in HA synthesis and its receptor CD44 are expressed in cumulus oocyte complexes (COCs) in different animal species and increase during maturation. Hyaluronidase enzymes (Hyal) degrade HA into smaller biologically active HA fragments. To investigate the effects of the molecular size and concentration of HA on oocyte maturation and further embryo development, bovine oocytes were matured in vitro in the presence or absence of HA, Hyal-2 or 4-methylumbelliferone (4-MU); an HA synthesis inhibitor. The rates of oocyte nuclear maturation to metaphase II stage and development of embryos to blastocyst stage and blastocyst quality were recorded. Hyal-2 inhibited cumulus cell expansion without affecting oocyte maturation and further embryo development. Whereas, 4-MU at 1 mm reduced cumulus cell expansion, oocyte maturation rate and further embryo development; an effect which was partially abrogated by exogenous HA supplementation. These data suggest that HA production by cumulus cells during maturation is essential not only for cumulus cell expansion, but also for oocyte maturation and further embryo development. This effect is not affected by HA-degradation by Hyal-2.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | R Medicine > R Medicine (General) | ||||
| Divisions: | Faculty of Medicine > Warwick Medical School | ||||
| Journal or Publication Title: | Theriogenology | ||||
| Publisher: | Elsevier Inc. | ||||
| ISSN: | 0093-691X | ||||
| Official Date: | August 2012 | ||||
| Dates: |
|
||||
| Volume: | Vol.78 | ||||
| Number: | No.3 | ||||
| Page Range: | pp. 670-677 | ||||
| Identifier: | 10.1016/j.theriogenology.2012.03.013 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
