Erent, Muriel, Drummond, Douglas R. and Cross, Robert A.. (2012) S. pombe kinesins-8 promote both nucleation and catastrophe of microtubules. PLoS One, Vol.7 (No.2). e30738. ISSN 1932-6203

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Abstract

The kinesins-8 were originally thought to be microtubule depolymerases, but are now emerging as more versatile catalysts of microtubule dynamics. We show here that S. pombe Klp5-436 and Klp6-440 are non-processive plus-end-directed motors whose in vitro velocities on S. pombe microtubules at 7 and 23 nm s−1 are too slow to keep pace with the growing tips of dynamic interphase microtubules in living S. pombe. In vitro, Klp5 and 6 dimers exhibit a hitherto-undescribed combination of strong enhancement of microtubule nucleation with no effect on growth rate or catastrophe frequency. By contrast in vivo, both Klp5 and Klp6 promote microtubule catastrophe at cell ends whilst Klp6 also increases the number of interphase microtubule arrays (IMAs). Our data support a model in which Klp5/6 bind tightly to free tubulin heterodimers, strongly promoting the nucleation of new microtubules, and then continue to land as a tubulin-motor complex on the tips of growing microtubules, with the motors then dissociating after a few seconds residence on the lattice. In vivo, we predict that only at cell ends, when growing microtubule tips become lodged and their growth slows down, will Klp5/6 motor activity succeed in tracking growing microtubule tips. This mechanism would allow Klp5/6 to detect the arrival of microtubule tips at cells ends and to amplify the intrinsic tendency for microtubules to catastrophise in compression at cell ends. Our evidence identifies Klp5 and 6 as spatial regulators of microtubule dynamics that enhance both microtubule nucleation at the cell centre and microtubule catastrophe at the cell ends.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Medicine > Warwick Medical School > Biomedical Cell Biology
Library of Congress Subject Headings (LCSH):	Kinesin, Microtubules, Schizosaccharomyces pombe -- Physiology
Journal or Publication Title:	PLoS One
Publisher:	Public Library of Science
ISSN:	1932-6203
Date:	February 2012
Volume:	Vol.7
Number:	No.2
Page Range:	e30738
Identification Number:	10.1371/journal.pone.0030738
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Marie Curie Cancer Care, Medical Research Council (Great Britain) (MRC)
Grant number:	G0200542 (MRC)
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URI:	http://wrap.warwick.ac.uk/id/eprint/49405

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