CO2-dependent opening of connexin 26 and related beta connexins
Huckstepp, Robert T. R., Eason, Robert, Sachdev, Anshu and Dale, Nicholas. (2010) CO2-dependent opening of connexin 26 and related beta connexins. Journal of Physiology, Vol.588 (No.20). pp. 3921-3931. ISSN 0022-3751Full text not available from this repository.
Official URL: http://dx.doi.org/10.1113/jphysiol.2010.192096
We have previously shown connexin mediated CO2-dependent ATP release from the surface of the medulla oblongata. Given the localization of connexin 26 (Cx26) to the chemosensing areas of the medulla, we have tested in a heterologous expression system (HeLa cells) whether Cx26 may be sensitive to changes in P-CO2. Cx26 responded to an increase in P-CO2 at constant extracellular pH by opening and to a decrease in P-CO2 by closing. Furthermore, Cx26 was partially activated at a physiological P-CO2 of around 40 mmHg. Cx26 in isolated patches responded to changes in P-CO2, suggesting direct CO2 sensitivity of the hemichannel to CO2. Heterologous expression of Cx26 in HeLa cells was sufficient to endow them with the capacity to release ATP in a CO2-sensitive manner. We have examined other heterologously expressed connexins for their ability to respond to changes in P-CO2. The closely related beta connexins Cx30 and Cx32 also displayed sensitivity to changes in P-CO2, but with slightly different characteristics from Cx26. The more distant Cx43 exhibited CO2-dependent closing (possibly mediated through intracellular acidification), while Cx36 displayed no CO2 sensitivity. These surprising findings suggest that connexins may play a hitherto unappreciated variety of signalling roles, and that Cx26 and related beta connexins may impart direct sensitivity to CO2 throughout the brain.
|Item Type:||Journal Article|
|Subjects:||R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Q Science > QP Physiology
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Journal or Publication Title:||Journal of Physiology|
|Publisher:||Wiley-Blackwell Publishing Ltd.|
|Date:||15 October 2010|
|Number of Pages:||11|
|Page Range:||pp. 3921-3931|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Medical Research Council (Great Britain) (MRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)|
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