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Arabidopsis defense against Botrytis cinerea : chronology and regulation deciphered by high-resolution temporal transcriptomic analysis
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(2012) Arabidopsis defense against Botrytis cinerea : chronology and regulation deciphered by high-resolution temporal transcriptomic analysis. The Plant Cell, Vol.24 (No.9). pp. 3530-3557. doi:10.1105/tpc.112.102046 ISSN 1040-4651.
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WRAP_Denby_0581786-lf-280912-plant_cell-2012-windram-tpc.112.102046.pdf - Published Version Download (3139Kb) | Preview |
Official URL: http://www.plantcell.org/content/early/2012/09/27/...
Abstract
Transcriptional reprogramming forms a major part of a plant’s response to pathogen infection. Many individual components and pathways operating during plant defense have been identified, but our knowledge of how these different components interact is still rudimentary. We generated a high-resolution time series of gene expression profiles from a single Arabidopsis thaliana leaf during infection by the necrotrophic fungal pathogen Botrytis cinerea. Approximately one-third of the Arabidopsis genome is differentially expressed during the first 48 h after infection, with the majority of changes in gene expression occurring before significant lesion development. We used computational tools to obtain a detailed chronology of the defense response against B. cinerea, highlighting the times at which signaling and metabolic processes change, and identify transcription factor families operating at different times after infection. Motif enrichment and network inference predicted regulatory interactions, and testing of one such prediction identified a role for TGA3 in defense against necrotrophic pathogens. These data provide an unprecedented level of detail about transcriptional changes during a defense response and are suited to systems biology analyses to generate predictive models of the gene regulatory networks mediating the Arabidopsis response to B. cinerea.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QK Botany | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Research Centres > Molecular Organisation and Assembly in Cells (MOAC) Faculty of Science, Engineering and Medicine > Science > Statistics Faculty of Science, Engineering and Medicine > Research Centres > Warwick Systems Biology Centre |
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| Library of Congress Subject Headings (LCSH): | Arabidopsis thaliana -- Physiology, Arabidopsis thaliana -- Disease and pest resistance, Arabidopsis thaliana -- Defenses, Botrytis cinerea, Plant genetic regulation | ||||
| Journal or Publication Title: | The Plant Cell | ||||
| Publisher: | American Society of Plant Biologists | ||||
| ISSN: | 1040-4651 | ||||
| Official Date: | September 2012 | ||||
| Dates: |
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| Volume: | Vol.24 | ||||
| Number: | No.9 | ||||
| Page Range: | pp. 3530-3557 | ||||
| DOI: | 10.1105/tpc.112.102046 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||
| Date of first compliant deposit: | 22 December 2015 | ||||
| Date of first compliant Open Access: | 22 December 2015 | ||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Engineering and Physical Sciences Research Council (EPSRC), L'Oréal (Firm), Unesco, National Science Foundation (U.S.) (NSF) | ||||
| Grant number: | BB/F005806/1 (BBSRC), 1021861 (NSF) |
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