Leszczyszyn, Oksana I. and Blindauer, Claudia A.. (2010) Zinc transfer from the embryo-specific metallothionein E-C from wheat : a case study. Physical Chemistry Chemical Physics, Vol.12 (No.41). pp. 13408-13418. ISSN 1463-9076

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Abstract

The direct observation of binding and release of spectroscopically silent metal ions such as Zn2+ and Ca2+ by proteins has been challenging before the advent of native electrospray ionisation mass spectrometry. This report highlights the powerful capability of ESI-MS to provide insight into metalloprotein speciation that is independent of any spectroscopic property. Using the zinc-binding plant metallothionein E-C from wheat as a study case, we show that ESI-MS is unique amongst other techniques in capturing intermediary metallospecies that evolve during the course of metal transfer to the chelator EDTA, as a model reaction to mimic the biological function of the protein as a zinc donor. Zinc release from the two-domain protein E-C appears to be extremely rapid and non-cooperative, and progresses with loss of one zinc ion from the fully loaded Zn-6 species, and a transient build-up of Zn-5 and Zn-4 species, which further react to give species with 0-3 zinc ions bound. H-1 NMR data has provided further insights into the different behaviour of the two domains upon metal depletion.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QK Botany
Divisions:	Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH):	Metallothionein, Zinc-finger proteins, Zinc -- Physiological effect, Plants -- Effect of zinc on, Metal ions, Electrospray ionization mass spectrometry
Journal or Publication Title:	Physical Chemistry Chemical Physics
Publisher:	Royal Society of Chemistry
ISSN:	1463-9076
Date:	2010
Volume:	Vol.12
Number:	No.41
Number of Pages:	11
Page Range:	pp. 13408-13418
Identification Number:	10.1039/c0cp00680g
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Royal Society (Great Britain), Engineering and Physical Sciences Research Council (EPSRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)
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URI:	http://wrap.warwick.ac.uk/id/eprint/5015

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