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Zinc transfer from the embryo-specific metallothionein E-C from wheat : a case study
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Leszczyszyn, Oksana I. and Blindauer, Claudia A. (2010) Zinc transfer from the embryo-specific metallothionein E-C from wheat : a case study. Physical Chemistry Chemical Physics, Vol.12 (No.41). pp. 13408-13418. doi:10.1039/c0cp00680g ISSN 1463-9076.
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Official URL: http://dx.doi.org/10.1039/c0cp00680g
Abstract
The direct observation of binding and release of spectroscopically silent metal ions such as Zn2+ and Ca2+ by proteins has been challenging before the advent of native electrospray ionisation mass spectrometry. This report highlights the powerful capability of ESI-MS to provide insight into metalloprotein speciation that is independent of any spectroscopic property. Using the zinc-binding plant metallothionein E-C from wheat as a study case, we show that ESI-MS is unique amongst other techniques in capturing intermediary metallospecies that evolve during the course of metal transfer to the chelator EDTA, as a model reaction to mimic the biological function of the protein as a zinc donor. Zinc release from the two-domain protein E-C appears to be extremely rapid and non-cooperative, and progresses with loss of one zinc ion from the fully loaded Zn-6 species, and a transient build-up of Zn-5 and Zn-4 species, which further react to give species with 0-3 zinc ions bound. H-1 NMR data has provided further insights into the different behaviour of the two domains upon metal depletion.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry Q Science > QK Botany |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Library of Congress Subject Headings (LCSH): | Metallothionein, Zinc-finger proteins, Zinc -- Physiological effect, Plants -- Effect of zinc on, Metal ions, Electrospray ionization mass spectrometry | ||||
Journal or Publication Title: | Physical Chemistry Chemical Physics | ||||
Publisher: | Royal Society of Chemistry | ||||
ISSN: | 1463-9076 | ||||
Official Date: | 2010 | ||||
Dates: |
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Volume: | Vol.12 | ||||
Number: | No.41 | ||||
Number of Pages: | 11 | ||||
Page Range: | pp. 13408-13418 | ||||
DOI: | 10.1039/c0cp00680g | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Royal Society (Great Britain), Engineering and Physical Sciences Research Council (EPSRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) |
Data sourced from Thomson Reuters' Web of Knowledge
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