Fragrance chemicals lyral and lilial decrease viability of HaCat cells’ by increasing free radical production and lowering intracellular ATP level : protection by antioxidants
Usta, Julnar, Hachem, Yassmine, El-Rifai, Omar, Bou-Moughlabey, Yolla, Echtay, Karim, Griffiths, David, Nakkash-Chmaisse, Hania and Makki, Rajaa Fakhoury. (2013) Fragrance chemicals lyral and lilial decrease viability of HaCat cells’ by increasing free radical production and lowering intracellular ATP level : protection by antioxidants. Toxicology in Vitro, Volume 27 (Number 1). pp. 339-348. ISSN 0887-2333Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.tiv.2012.08.020
We investigate in this study the biochemical effects on cells in culture of 2 commonly used fragrance chemicals: lyral and lilial. Whereas both chemicals exerted a significant effect on primary keratinocyte (s), HaCat cells, no effect was obtained with any of HepG2, Hek293, Caco2, NIH3T3, and MCF7 cells. Lyral and lilial: a) decreased the viability of HaCat cells with a 50% cell death at 100nM and 60nM respectively; b) decreased significantly in a dose dependant manner the intracellular ATP level following 12-hrs of treatment; c) inhibited complexes I and II of electron transport chain in liver sub-mitochondrial particles; d) increased reactive oxygen species generation that was reversed by N-acetyl cysteine and trolox and the natural antioxidant lipoic acid, without influencing the level of free and/or oxidized glutathione. Lipoic acid protected HaCat cells against the decrease in viability induced by either compound. Dehydrogenation of lyral and lilial produce Î±-Î²-unsaturated aldehydes, that reacts with lipoic acid requiring proteins resulting in their inhibition. We propose lyral and lilial as toxic to mitochondria that have a direct effect on electron transport chain, increase ROS production, derange mitochondrial membrane potential, and decrease cellular ATP level, leading thus to cell death. âº Fragrant chemicals Lyral /lilial decreased viability of HaCat cells. âº Lyral / lilial- decreased ATP level and increased: ROS level and LDH release in HaCat cells. âº Lyral /lilial inhibited liver sub-mitochondrial particles complexes I and II activities of respiratory chain. âº Antioxidants restored control ROS level; lipoic acid protected the viability of lilial and lyral treated Hacat cells. âº Lyral and lilila are mitochondriotoxin with direct and indirect effect on HaCat cells.
|Item Type:||Journal Article|
|Divisions:||Faculty of Science > Chemistry|
|Journal or Publication Title:||Toxicology in Vitro|
|Official Date:||February 2013|
|Page Range:||pp. 339-348|
|Access rights to Published version:||Restricted or Subscription Access|
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