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Autophagic degradation of dBruce controls DNA fragmentation in nurse cells during late Drosophila melanogaster oogenesis
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Nezis, I. P., Shravage, Bhupendra V., Sagona, Antonia P., Lamark, Trond, Bjorkoy, G., Johansen, Terje, Rusten, Tor Erik, Brech, Andreas, Baehrecke, Eric H. and Stenmark, H. (Harald) (2010) Autophagic degradation of dBruce controls DNA fragmentation in nurse cells during late Drosophila melanogaster oogenesis. The Journal of Cell Biology, Vol. 190 (No. 4). pp. 523-531. doi:10.1083/jcb.201002035 ISSN 0021-9525.
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Official URL: http://dx.doi.org/10.1083/jcb.201002035
Abstract
Autophagy is an evolutionarily conserved pathway responsible for degradation of cytoplasmic material via the lysosome. Although autophagy has been reported to contribute to cell death, the underlying mechanisms remain largely unknown. In this study, we show that autophagy controls DNA fragmentation during late oogenesis in Drosophila melanogaster. Inhibition of autophagy by genetically removing the function of the autophagy genes atg1, atg13, and vps34 resulted in late stage egg chambers that contained persisting nurse cell nuclei without fragmented DNA and attenuation of caspase-3 cleavage. The Drosophila inhibitor of apoptosis (IAP) dBruce was found to colocalize with the autophagic marker GFP-Atg8a and accumulated in autophagy mutants. Nurse cells lacking Atg1 or Vps34 in addition to dBruce contained persisting nurse cell nuclei with fragmented DNA. This indicates that autophagic degradation of dBruce controls DNA fragmentation in nurse cells. Our results reveal autophagic degradation of an IAP as a novel mechanism of triggering cell death and thereby provide a mechanistic link between autophagy and cell death.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | The Journal of Cell Biology | ||||
Publisher: | The Rockefeller University Press | ||||
ISSN: | 0021-9525 | ||||
Official Date: | 2010 | ||||
Dates: |
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Volume: | Vol. 190 | ||||
Number: | No. 4 | ||||
Page Range: | pp. 523-531 | ||||
DOI: | 10.1083/jcb.201002035 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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