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PtdIns(3)P controls cytokinesis through KIF13A-mediated recruitment of FYVE-CENT to the midbody
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Sagona, Antonia P., Nezis, I. P., Pedersen, Nina Marie, Liestøl, Knut, Poulton, John, Rusten, Tor Erik, Skotheim, Rolf I., Raiborg, Camilla and Stenmark, H. (Harald) (2010) PtdIns(3)P controls cytokinesis through KIF13A-mediated recruitment of FYVE-CENT to the midbody. Nature Cell Biology, Vol. 12 (No. 4). pp. 362-371. doi:10.1038/ncb2036 ISSN 1465-7392.
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Official URL: http://dx.doi.org/10.1038/ncb2036
Abstract
Several subunits of the class III phosphatidylinositol-3-OH kinase (PI(3)K-III) complex are known as tumour suppressors. Here we uncover a function for this complex and its catalytic product phosphatidylinositol-3-phosphate (PtdIns(3)P) in cytokinesis. We show that PtdIns(3)P localizes to the midbody during cytokinesis and recruits a centrosomal protein, FYVE-CENT (ZFYVE26), and its binding partner TTC19, which in turn interacts with CHMP4B, an endosomal sorting complex required for transport (ESCRT)-III subunit implicated in the abscission step of cytokinesis. Translocation of FYVE-CENT and TTC19 from the centrosome to the midbody requires another FYVE-CENT-interacting protein, the microtubule motor KIF13A. Depletion of the VPS34 or Beclin 1 subunits of PI(3)K-III causes cytokinesis arrest and an increased number of binucleate and multinucleate cells, in a similar manner to the depletion of FYVE-CENT, KIF13A or TTC19. These results provide a mechanism for the translocation and docking of a cytokinesis regulatory machinery at the midbody.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Nature Cell Biology | ||||
Publisher: | Nature Publishing Group | ||||
ISSN: | 1465-7392 | ||||
Official Date: | 2010 | ||||
Dates: |
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Volume: | Vol. 12 | ||||
Number: | No. 4 | ||||
Page Range: | pp. 362-371 | ||||
DOI: | 10.1038/ncb2036 | ||||
Status: | Not Peer Reviewed | ||||
Access rights to Published version: | Restricted or Subscription Access |
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