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Cautionary Notes on the Use of C-Terminal BAK1 Fusion Proteins for Functional Studies
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Ntoukakis, Vardis, Schwessinger, B., Segonzac, C. and Zipfel, C. (2011) Cautionary Notes on the Use of C-Terminal BAK1 Fusion Proteins for Functional Studies. The Plant Cell, Vol. 23 (No. 11). pp. 3871-3878. doi:10.1105/tpc.111.090779 ISSN 1040-4651.
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Official URL: http://dx.doi.org/10.1105/tpc.111.090779
Abstract
Detailed phenotypic characterization reveals that several BAK1 fusion proteins with C-terminal tags strongly impair complementation of bak1 null mutants with respect to responsiveness to the bacterial pathogen-associated molecular patterns flagellin and EF-Tu. This raises concerns about the widespread use of such protein variants of this important regulatory Leu-rich repeat receptor-like kinase (RLK) for functional analyses of RLK-based signaling.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | The Plant Cell | ||||
| Publisher: | American Society of Plant Biologists | ||||
| ISSN: | 1040-4651 | ||||
| Official Date: | 2011 | ||||
| Dates: |
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| Volume: | Vol. 23 | ||||
| Number: | No. 11 | ||||
| Page Range: | pp. 3871-3878 | ||||
| DOI: | 10.1105/tpc.111.090779 | ||||
| Status: | Peer Reviewed | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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