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The mutualistic fungus Piriformospora indica colonizes Arabidopsis roots by inducing an endoplasmic reticulum stress-triggered caspase-dependent cell death
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Qiang, X., Zechmann, B., Reitz, M. U., Kogel, Karl-Heinz and Schäfer, Patrick (2012) The mutualistic fungus Piriformospora indica colonizes Arabidopsis roots by inducing an endoplasmic reticulum stress-triggered caspase-dependent cell death. The Plant Cell, Volume 24 (Number 2). pp. 794-809. doi:10.1105/tpc.111.093260
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WRAP_1172019-lf-270814-qiang12_p._indica_and_er-pcd_plant_cell.pdf - Accepted Version - Requires a PDF viewer. Download (3697Kb) | Preview |
Official URL: http://dx.doi.org/10.1105/tpc.111.093260
Abstract
In Arabidopsis thaliana roots, the mutualistic fungus Piriformospora indica initially colonizes living cells, which die as the colonization proceeds. We aimed to clarify the molecular basis of this colonization-associated cell death. Our cytological analyses revealed endoplasmic reticulum (ER) swelling and vacuolar collapse in invaded cells, indicative of ER stress and cell death during root colonization. Consistent with this, P. indica–colonized plants were hypersensitive to the ER stress inducer tunicamycin. By clear contrast, ER stress sensors bZIP60 and bZIP28 as well as canonical markers for the ER stress response pathway, termed the unfolded protein response (UPR), were suppressed at the same time. Arabidopsis mutants compromised in caspase 1–like activity, mediated by cell death–regulating vacuolar processing enzymes (VPEs), showed reduced colonization and decreased cell death incidence. We propose a previously unreported microbial invasion strategy during which P. indica induces ER stress but inhibits the adaptive UPR. This disturbance results in a VPE/caspase 1–like-mediated cell death, which is required for the establishment of the symbiosis. Our results suggest the presence of an at least partially conserved ER stress–induced caspase-dependent cell death pathway in plants as has been reported for metazoans.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QK Botany | ||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||
| Library of Congress Subject Headings (LCSH): | Arabidopsis thaliana, Basidiomycetes, Endoplasmic reticulum, Cell death | ||||
| Journal or Publication Title: | The Plant Cell | ||||
| Publisher: | American Society of Plant Biologists | ||||
| ISSN: | 1040-4651 | ||||
| Official Date: | February 2012 | ||||
| Dates: |
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| Date of first compliant deposit: | 23 December 2015 | ||||
| Volume: | Volume 24 | ||||
| Number: | Number 2 | ||||
| Page Range: | pp. 794-809 | ||||
| DOI: | 10.1105/tpc.111.093260 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Deutsche Forschungsgemeinschaft (DFG), Fonds zur Förderung der Wissenschaftlichen Forschung (Austria) (FWF) | ||||
| Grant number: | DFG-SPP1212 (DFG), P20619-B16 (FWF) |
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