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The mutualistic fungus Piriformospora indica colonizes Arabidopsis roots by inducing an endoplasmic reticulum stress-triggered caspase-dependent cell death

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Qiang, X., Zechmann, B., Reitz, M. U., Kogel, Karl-Heinz and Schäfer, Patrick (2012) The mutualistic fungus Piriformospora indica colonizes Arabidopsis roots by inducing an endoplasmic reticulum stress-triggered caspase-dependent cell death. The Plant Cell, Volume 24 (Number 2). pp. 794-809. doi:10.1105/tpc.111.093260

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Official URL: http://dx.doi.org/10.1105/tpc.111.093260

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Abstract

In Arabidopsis thaliana roots, the mutualistic fungus Piriformospora indica initially colonizes living cells, which die as the colonization proceeds. We aimed to clarify the molecular basis of this colonization-associated cell death. Our cytological analyses revealed endoplasmic reticulum (ER) swelling and vacuolar collapse in invaded cells, indicative of ER stress and cell death during root colonization. Consistent with this, P. indica–colonized plants were hypersensitive to the ER stress inducer tunicamycin. By clear contrast, ER stress sensors bZIP60 and bZIP28 as well as canonical markers for the ER stress response pathway, termed the unfolded protein response (UPR), were suppressed at the same time. Arabidopsis mutants compromised in caspase 1–like activity, mediated by cell death–regulating vacuolar processing enzymes (VPEs), showed reduced colonization and decreased cell death incidence. We propose a previously unreported microbial invasion strategy during which P. indica induces ER stress but inhibits the adaptive UPR. This disturbance results in a VPE/caspase 1–like-mediated cell death, which is required for the establishment of the symbiosis. Our results suggest the presence of an at least partially conserved ER stress–induced caspase-dependent cell death pathway in plants as has been reported for metazoans.

Item Type: Journal Article
Subjects: Q Science > QK Botany
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Arabidopsis thaliana, Basidiomycetes, Endoplasmic reticulum, Cell death
Journal or Publication Title: The Plant Cell
Publisher: American Society of Plant Biologists
ISSN: 1040-4651
Official Date: February 2012
Dates:
DateEvent
February 2012Published
Volume: Volume 24
Number: Number 2
Page Range: pp. 794-809
DOI: 10.1105/tpc.111.093260
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Deutsche Forschungsgemeinschaft (DFG), Fonds zur Förderung der Wissenschaftlichen Forschung (Austria) (FWF)
Grant number: DFG-SPP1212 (DFG), P20619-B16 (FWF)

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