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STARTS - A stable root transformation system for rapid functional analyses of proteins of the monocot model plant barley
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Imani, Jafargholi, Li, Liang, Schäfer, Patrick and Kogel, Karl-Heinz (2011) STARTS - A stable root transformation system for rapid functional analyses of proteins of the monocot model plant barley. The Plant Journal, Vol.67 (No.4). pp. 726-735. doi:10.1111/j.1365-313X.2011.04620.x ISSN 0960-7412.
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Official URL: http://dx.doi.org/10.1111/j.1365-313X.2011.04620.x
Abstract
Large data sets are generated from plants by the various ‘omics platforms. Currently, a limiting step in data analysis is the assessment of protein function and its translation into a biological context. The lack of robust high-throughput transformation systems for monocotyledonous plants, to which the vast majority of crop plants belong, is a major restriction and impedes exploitation of novel traits in agriculture. Here we present a stable root transformation system for barley, termed STARTS, that allows assessment of gene function in root tissues within 6 weeks. The system is based on the finding that a callus, produced on root induction medium from the scutellum of the immature embryo, is able to regenerate roots from single transformed cells by concomitant suppression of shoot development. Using Agrobacterium tumefaciens-mediated transfer of genes involved in root development and pathogenesis, we show that those calli regenerate large amounts of uniformly transformed roots for in situ functional analysis of newly expressed proteins.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | The Plant Journal | ||||
Publisher: | John Wiley & Sons Ltd. | ||||
ISSN: | 0960-7412 | ||||
Official Date: | August 2011 | ||||
Dates: |
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Volume: | Vol.67 | ||||
Number: | No.4 | ||||
Page Range: | pp. 726-735 | ||||
DOI: | 10.1111/j.1365-313X.2011.04620.x | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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