Eastmond, Peter J., Quettier, Anne-Laure, Kroon, Johan T. M., Craddock, Christian, Adams, Nicolette and Slabas, Antoni R.. (2010) Phosphatidic acid phosphohydrolase1 and 2 regulate phospholipid synthesis at the endoplasmic reticulum in arabidopsis. Plant Cell, Vol.22 (No.8). pp. 2796-2811. ISSN 1040-4651

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Abstract

Phospholipid biosynthesis is essential for the construction of most eukaryotic cell membranes, but how this process is regulated in plants remains poorly understood. Here, we show that in Arabidopsis thaliana, two Mg2+-dependent phosphatidic acid phosphohydrolases called PAH1 and PAH2 act redundantly to repress phospholipid biosynthesis at the endoplasmic reticulum (ER). Leaves from pah1 pah2 double mutants contain similar to 1.8-fold more phospholipid than the wild type and exhibit gross changes in ER morphology, which are consistent with massive membrane overexpansion. The net rate of incorporation of [methyl-C-14]choline into phosphatidylcholine (PC) is similar to 1.8-fold greater in the double mutant, and the transcript abundance of several key genes that encode enzymes involved in phospholipid synthesis is increased. In particular, we show that PHOSPHORYLETHANOLAMINE N-METHYLTRANSFERASE1 (PEAMT1) is upregulated at the level of transcription in pah1 pah2 leaves. PEAMT catalyzes the first committed step of choline synthesis in Arabidopsis and defines a variant pathway for PC synthesis not found in yeasts or mammals. Our data suggest that PAH1/2 play a regulatory role in phospholipid synthesis that is analogous to that described in Saccharomyces cerevisiae. However, the target enzymes differ, and key components of the signal transduction pathway do not appear to be conserved.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology Q Science > QK Botany Q Science > QP Physiology S Agriculture > SB Plant culture
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Phosphatidic acids, Phosphatases, Phospholipids, Endoplasmic reticulum, Arabidopsis, Biochemistry
Journal or Publication Title:	Plant Cell
Publisher:	American Society of Plant Biologists
ISSN:	1040-4651
Official Date:	August 2010
Volume:	Vol.22
Number:	No.8
Number of Pages:	16
Page Range:	pp. 2796-2811
Identification Number:	10.1105/tpc.109.071423
Status:	Peer Reviewed
Publication Status:	Published
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)
URI:	http://wrap.warwick.ac.uk/id/eprint/5124

Data sourced from Thomson Reuters' Web of Knowledge

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