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RNA-directed DNA methylation and plant development require an IWR1-type transcription factor
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Kanno, Tatsuo, Bucher, Etienne, Daxinger, Lucia, Huettel, Bruno, Kreil, David, Breinig, Frank, Lind, Marc, Schmitt, Manfred J, Simon, Stacey A, Gurazada, Sai Guna Ranjan, Meyers, Blake C, Lorkovic, Zdravko J, Matzke, Antonius J M and Matzke, Marjori (2010) RNA-directed DNA methylation and plant development require an IWR1-type transcription factor. EMBO reports, Vol.11 (No.1). pp. 65-71. doi:10.1038/embor.2009.246 ISSN 1469-221X.
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Official URL: http://dx.doi.org/10.1038/embor.2009.246
Abstract
RNA-directed DNA methylation (RdDM) in plants requires two RNA polymerase (Pol) II-related RNA polymerases, namely Pol IV and Pol V. A genetic screen designed to reveal factors that are important for RdDM in a developmental context in Arabidopsis identified DEFECTIVE IN MERISTEM SILENCING 4 (DMS4). Unlike other mutants defective in RdDM, dms4 mutants have a pleiotropic developmental phenotype. The DMS4 protein is similar to yeast IWR1 (interacts with RNA polymerase II), a conserved putative transcription factor that interacts with Pol II subunits. The DMS4 complementary DNA partly complements the K1 killer toxin hypersensitivity of a yeast iwr1 mutant, suggesting some functional conservation. In the transgenic system studied, mutations in DMS4 directly or indirectly affect Pol IV-dependent secondary short interfering RNAs, Pol V-mediated RdDM, Pol V-dependent synthesis of intergenic non-coding RNA and expression of many Pol II-driven genes. These data suggest that DMS4 might be a regulatory factor for several RNA polymerases, thus explaining its diverse roles in the plant.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | EMBO reports | ||||
Publisher: | Nature Publishing Group | ||||
ISSN: | 1469-221X | ||||
Official Date: | January 2010 | ||||
Dates: |
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Volume: | Vol.11 | ||||
Number: | No.1 | ||||
Page Range: | pp. 65-71 | ||||
DOI: | 10.1038/embor.2009.246 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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