Coelho, Paula S., Vicente, Joana G., Monteiro, António A. and Holub, Eric B.. (2012) Pathotypic diversity of Hyaloperonospora brassicae collected from Brassica oleracea. European Journal of Plant Pathology, Vol.134 (No.4). pp. 763-771. ISSN 0929-1873

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Abstract

Downy mildew caused by Hyaloperonospora brassicae is an economically destructive disease of brassica crops in many growing regions throughout the world. Specialised pathogenicity of downy mildews from different Brassica species and closely related ornamental or wild relatives has been described from host range studies. Pathotypic variation amongst Hyaloperonospora brassicae isolates from Brassica oleracea has also been described; however, a standard set of B. oleracea lines that could enable reproducible classification of H. brassicae pathotypes was poorly developed. For this purpose, we examined the use of eight genetically refined host lines derived from our previous collaborative work on downy mildew resistance as a differential set to characterise pathotypes in the European population of H. brassicae. Interaction phenotypes for each combination of isolate and host line were assessed following drop inoculation of cotyledons and a spectrum of seven phenotypes was observed based on the level of sporulation on cotyledons and visible host responses. Two host lines were resistant or moderately resistant to the entire collection of isolates, and another was universally susceptible. Five lines showed differential responses to the H. brassicae isolates. A minimum of six pathotypes and five major effect resistance genes are proposed to explain all of the observed interaction phenotypes. The B. oleracea lines from this study can be useful for monitoring pathotype frequencies in H. brassicae populations in the same or other vegetable growing regions, and to assess the potential durability of disease control from different combinations of the predicted downy mildew resistance genes.

Item Type:	Journal Article
Subjects:	S Agriculture > SB Plant culture
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Downy mildew diseases -- Pathogenesis, Cole crops -- Diseases and pests, Cole crops -- Genetics
Journal or Publication Title:	European Journal of Plant Pathology
Publisher:	Springer
ISSN:	0929-1873
Date:	December 2012
Volume:	Vol.134
Number:	No.4
Page Range:	pp. 763-771
Identification Number:	10.1007/s10658-012-0052-z
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Fundação para a Ciência e a Tecnologia (FCT), Great Britain. Dept. for Environment, Food & Rural Affairs (DEFRA)
Grant number:	PTDC/AGR-GPL/70135/2006 (FCT), HH37232SX (DEFRA)
References:	Agnola, B., Boury, S., Monot, C., Quillévéré, A., Hervé, Y. & Silué, D. (2003). Evidence that a leaf-disk test allows assessment of isolate-specific resistance in Brassica oleracea crops against downy mildew (Peronospora parasitica). European Journal Plant Pathology, 109, 471-478. Armstrong, M. R., Whisson, S. C., Pritchard, L., Bos, J. I. B., Venter, E., Avrova, A. O., Rehmany, A. P., Böhme, U., Brooks, K., Cherevach, I., Hamlin, N., White, B., Fraser, A., Lord, A., Quail, M. A., Churcher, C., Hall, N., Berriman, M., Huang, S., Kamoun, S., Beynon, J. L. & Birch, P. R. J. (2005). An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognized in the host cytoplasm. Proceedings of the National Academy of Sciences USA, 102, 7766-7771. Bahcevandziev, K. (2003). Caracterização da resistência ao míldio (Peronospora parasitica (Pers. Ex. Fr.) Fr.) no estado cotiledonar em Brassica oleracea. Dissertation, Instituto Superior de Agronomia, Technical University of Lisbon. Channon, A. G. (1981). Downy mildew of brassicas. In D. M. Spencer (Ed.), The downy mildews (pp. 321-339). London: Academic Press. Coelho, P., Leckie, D., Bahcevandziev, K., Valério, L., Astley, D., Boukema, I., Crute, I. R. & Monteiro, A. (1998). The relationship between cotyledon and adult plant resistance to downy mildew (Peronospora parasitica) in Brassica oleracea. Acta Horticulturae, 459, 335-342. Correll, J. C., Feng, C., Irish, B. M., Koike, S. T., Morelock, T. E., Bentley, T. C. & Tomlinson, A. N. (2007). Spinach downy mildew: overview of races and the development of molecular markers linked to major resistance genes. In A. Lebeda & P.T. N. Spencer-Phillips (Eds.), Advances in downy mildew research (pp. 135-142). Olomouc: Organising Committee of the 2nd International Downy Mildews Symposium. Crute, I. R. & Johnson, A. G. (1976). The genetic relationship between races of Bremia lactucae and cultivares of Lactuca sativa. Annals of Applied Biology, 83, 125-137. Delmotte, F., Giresse, X., Richard-Cervera, S., M'Baya, J., Vear, F., Tourvieille, J., Walser, P. & Labrouhe, D. T. (2008). Single nucleotide polymorphisms reveal multiple introductions into France of Plasmopara halstedii, the plant pathogen causing sunflower downy mildew. Infection, Genetics and Evolution, 8, 534-540. Dickinson, C. H. & Greenhalgh, J. R. (1977). Host range and taxonomy of Peronospora on crucifers. Transactions of the British Mycological Society, 69, 111-116. Dickson, M. H. & Petzoldt, R. (1993). Plant age and isolate source affect expression of downy mildew resistance in broccoli. HortScience, 28, 730-731. Göker, M., Voglmayr, H., Blázquez, G. G. & Oberwinkler, F. (2009). Species delimitation in downy mildews: the case of Hyaloperonospora in the light of nuclear ribosomal ITS and LSU sequences. Mycological Research, 113, 308-325. Greenhalgh, J. R. & Dickinson, C. H. (1975). Differential reactions of three crucifers to infection by Peronospora parasitica (Pers. ex Fr.) Fr.. Phytopathologische Zeitschrift – Journal of Phytopathology, 84, 131-141. Holub, E. B. (2008). Natural history of Arabidopsis thaliana and oomycete symbioses. European Journal of Plant Pathology, 122: 91-109. Hott, T. W., Durgan, M. E. & Michelmore, R. W. (1987). Genetics of virulence in Californian populations of Bremia lactucae (lettuce downy mildew). Phytopathology, 77, 1381-1386. T. W., Hulbert, S. H. & Michelmore, R. W. (1989). Genetic analysis of the gene-for-gene interaction between lettuce (Lactuca sativa) and Bremia lactucae. Phytopathology, 79, 888-897. Hulbert, S. H. & Michelmore, R. W. (1985). Linkage analysis of genes for resistance to downy mildew (Bremia lactucae) in lettuce (Lactuca sativa). Theoretical and Applied Genetics, 70, 520-528. Irish, B. M., Correll, J. C., Koike, S. T., Schafer, J. & Morelock, T. E. (2003). Identification and cultivar reaction to three new races of the spinach downy mildew pathogen from the United States and Europe. Plant Disease, 87, 567-572. Jensen, B. D., Hockenhull, J. & Munk, L. (1999). Seedling and adult plant resistance to downy mildew (Peronospora parasitica) in cauliflower (Brassica oleracea convar. botrytis var. botrytis). Plant Pathology, 48, 604-612. Leckie, D., Astley, D., Crute, I. R., Ellis, P. R., Pink, D. A. C., Boukema, I., Monteiro, A. A. & Dias, S. (1996). The location and exploitation of genes for pest and disease resistance in European gene bank collections of horticultural brassicas. Acta Horticulturae, 407, 95-101. Leckie, D., Cogan, N., Astley, D., Crute, I. R., Boukema, I., Santos, M., Bahcevandziev, K., Silva-Dias, J. & Monteiro, A. A. (1998). Differential resistance to Peronospora parasitica and Albugo candida in Brassica oleracea. Acta Horticulturae, 459, 357-361. Lucas, J. A., Crute, I. R., Sherriff, C. & Gordon, P. L. (1988). The identification of a gene for race-specific resistance to Peronospora parasitica (downy mildew) in Brassica napus var. oleifera (oilseed rape). Plant Pathology, 37, 538-545. MacGregor, T., Bhattacharyya, M., Tyler, B., Bhat, R., Schmitthenner, A. F. & Gijzen, M. (2002). Genetic and physical mapping of Avr1a in Phytophthora sojae. Genetics, 160, 949-959. Monot, C. & Silué, D. (2009). Comparison of the resistance and susceptibility to downy mildew [Hyaloperonospora parasitica, constant. (Pers. Ex Fr)] of nine Brassica oleracea accessions in laboratory, seedbed, and field screens. Euphytica, 169, 413-419. Monteiro, A. A., Coelho, P. S., Bahcevandziev, K. & Valério, L. (2005). Inheritance of downy mildew resistance at cotyledon and adult-plant stages in ‘Couve Algarvia’ (Brassica oleracea var. tronchuda). Euphytica, 141, 85-92. Moss, N. A., Crute, I. R. & Lucas, J. A. (1994). Laboratory production of oospores of Peronospora parasitica (crucifer downy mildew) and the recovery and characterization of sexual progeny from crosses between isolates with different host specificity. Plant Pathology, 43, 713-725. Moss, N. A., Lucas, J. A. & Crute, I. R. (1991a). Evidence for differential response to isolates of Peronospora parasitica (downy mildew) in Brassica rapa. Annals of Applied Biology, 118, 96-97. Moss, N. A., Lucas, J. A., Crute, I. R. & Gordon, P. L. (1991b). Sources of seedling resistance to Peronospora parasitica (Downy mildew) in Brassica oleracea. Annals of Applied Biology, 118, 94-95. Nashaat, N. I. & Awasthi, R. P. (1995). Evidence for differential resistance to Peronospora parasitica (downy mildew) in accessions of Brassica juncea (mustard) at the cotyledon stage. Journal of Phytopathology, 143, 157-159. Nashaat, N. I. & Rawlinson, C. J. (1994). The response of oilseed rape (Brassica napus ssp. oleifera) accessions with different glucosinolate and erucic acid contents to four isolates of Peronospora parasitica (downy mildew) and the identification of new sources of resistance. Plant Pathology, 43, 278-285. Natti, J. J., Dickson, M. H. & Atkin, J. D. (1967). Resistance of Brassica oleracea varieties to downy mildew. Phytopathology, 57, 144-147. Norwood, J. M. & Crute, I. R. (1984). The genetic control and expression of specificity in Bremia lactucae (lettuce downy mildew). Plant Pathology, 33, 385-400. Shan, W., Cao, M., Leung, D. & Tyler, B. M. (2004). The Avr1b locus of Phytophthora sojae encodes an elicitor and a regulator required for avirulence on soybean plants carrying resistance gene Rps1b. Molecular Plant-Microbe Interactions, 17, 394-403. Sherriff, C. & Lucas, J. A. (1990). The host range of isolates of downy mildew, Peronospora parasitica, from Brassica crop species. Plant Pathology, 39, 77-91. Silué, D., Nashaat, N. I. & Tirilly, Y. (1996). Differential responses of Brassica oleracea and B. rapa accessions to seven isolates of Peronospora parasitica at the cotyledon stage. Plant Disease, 80, 142-144. Tapsoba, H. & Wilson, J. P. (1996). Pathogenic variation in Puccinia substriata var. indica in the Southeastern United States and screening for resistance in pearl millet germ plasm. Plant Disease, 80, 395-397. Thomas, C. E. & Jourdain, E. L. (1992). Resistance to race 2 of Peronospora parasitica in U.S. plant introductions of Brassica oleracea var. capitata. HortScience, 27, 1120-1122. Vicente, J. G., Gunn, N. D., Bailey, L., Pink, D. A. C. & Holub E. B. (2012). Genetics of resistance to downy mildew in Brassica oleracea and breeding towards durable disease control for UK vegetable production. Plant Pathology, 61, 600-609. Wang, M., Farnham, M. W. & Thomas, C. E. (2000). Phenotypic variation for downy mildew resistance among inbred broccoli. HortScience, 35, 925-929.
URI:	http://wrap.warwick.ac.uk/id/eprint/51740

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