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Structure and Molecular Mechanism of a Nucleobase-Cation-Symport-1 Family Transporter
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Weyand, Simone, Shimamura, Tatsuro, Yajima, S., Suzuki, S., Mirza, O., Krusong, K., Carpenter, E. P., Rutherford, N. G., Hadden, J. M., O'Reilly, J., Ma, P., Saidijam, M., Patching, S. G., Hope, R. J., Norbertczak, H. T., Roach, P. C. J., Iwata, So, Henderson, P. J. F. (Peter J. F.) and Cameron, Alexander (2008) Structure and Molecular Mechanism of a Nucleobase-Cation-Symport-1 Family Transporter. Science, Vol. 322 (No. 5902). pp. 709-713. doi:10.1126/science.1164440 ISSN 0036-8075.
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Official URL: http://dx.doi.org/10.1126/science.1164440
Abstract
The nucleobase–cation–symport-1 (NCS1) transporters are essential components of salvage pathways for nucleobases and related metabolites. Here, we report the 2.85-angstrom resolution structure of the NCS1 benzyl-hydantoin transporter, Mhp1, from Microbacterium liquefaciens. Mhp1 contains 12 transmembrane helices, 10 of which are arranged in two inverted repeats of five helices. The structures of the outward-facing open and substrate-bound occluded conformations were solved, showing how the outward-facing cavity closes upon binding of substrate. Comparisons with the leucine transporter LeuTAa and the galactose transporter vSGLT reveal that the outward- and inward-facing cavities are symmetrically arranged on opposite sides of the membrane. The reciprocal opening and closing of these cavities is synchronized by the inverted repeat helices 3 and 8, providing the structural basis of the alternating access model for membrane transport.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Science | ||||
Publisher: | American Association for the Advancement of Science | ||||
ISSN: | 0036-8075 | ||||
Official Date: | 2008 | ||||
Dates: |
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Volume: | Vol. 322 | ||||
Number: | No. 5902 | ||||
Page Range: | pp. 709-713 | ||||
DOI: | 10.1126/science.1164440 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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