Mueller, Boje, Noll, Gundula A., Ernst, Antonia M., Rueping, Boris, Groscurth, Sira, Twyman, Richard M., Kawchuk, Lawrence M. and Pruefer, Dirk. (2010) Recombinant artificial forisomes provide ample quantities of smart biomaterials for use in technical devices. Applied Microbiology and Biotechnology, Vol.88 (No.3). pp. 689-698. ISSN 0175-7598

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Abstract

Forisomes are mechanoproteins that undergo ATP-independent contraction-expansion cycles triggered by divalent cations, pH changes, and electrical stimuli. Although native forisomes from Medicago truncatula comprise a number of subunits encoded by separate genes, here we show that at least two of those subunits (MtSEO1 and MtSEO4) can assemble into homomeric forisome bodies that are functionally similar to their native, multimeric counterparts. We expressed these subunits in plants and yeast, resulting in the purification of large quantities of artificial forisomes with unique characteristics depending on the expression platform. These artificial forisomes were able to contract and expand in vitro like native forisomes and could respond to electrical stimulation when immobilized between interdigital transducer electrodes. These results indicate that recombinant artificial forisomes with specific characteristics can be prepared in large amounts and used as components of microscale and nanoscale devices.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title:	Applied Microbiology and Biotechnology
Publisher:	Springer
ISSN:	0175-7598
Official Date:	October 2010
Volume:	Vol.88
Number:	No.3
Number of Pages:	10
Page Range:	pp. 689-698
Identification Number:	10.1007/s00253-010-2771-4
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Bundesministerium fur Forschung und Bildung, Fraunhofer MAVO program, VolkswagenStiftung
Grant number:	0312014B, I/82 075
URI:	http://wrap.warwick.ac.uk/id/eprint/5199

Data sourced from Thomson Reuters' Web of Knowledge

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