Mori, Yumi, Purdy, Kevin J., Oakley, Brian B. and Kondo, Ryuji. (2010) Comprehensive detection of phototrophic sulfur bacteria using PCR primers that target reverse dissimilatory sulfite reductase gene. Microbes and Environments, Vol.25 (No.3). pp. 190-196. ISSN 1342-6311

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Abstract

A new set of primers for the detection of phototrophic sulfur bacteria in natural environments is described. The primers target the alpha-subunit of the reverse dissimilatory sulfite reductase gene (dsrA). PCR-amplification resulted in products of the expected size from all the phototrophic strains tested, including purple sulfur and green sulfur bacteria. Seventy-nine clones obtained from environmental DNA using the primers were sequenced and all found to be closely related to the dsrA of purple sulfur bacteria and green sulfur bacteria. This newly developed PCR assay targeting dsrA is rapid and simple for the detection of phototrophic sulfur bacteria in situ and superior to the use of culture-dependent techniques.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology Q Science > QR Microbiology
Divisions:	Faculty of Science > Life Sciences (2010- )
Journal or Publication Title:	Microbes and Environments
Publisher:	Japanese Soc Microbial Ecology, Dept Bioresource Science [Nihon Biseibutsu Seitai Gakkai]
ISSN:	1342-6311
Date:	3 September 2010
Volume:	Vol.25
Number:	No.3
Number of Pages:	7
Page Range:	pp. 190-196
Identification Number:	10.1264/jsme2.ME10109
Status:	Not Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Japan Society for the Promotion of Science, Fukui Prefectural Fund for the Promotion of Science, Kurita Water and Environment Foundation, EU Commission
Grant number:	MEXT-CT-2005-024112
URI:	http://wrap.warwick.ac.uk/id/eprint/5228

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