The Library
An in vivo control map for the eukaryotic mRNA translation machinery
Tools
Tools
Tools
Firczuk, Helena, Kannambath, Shichina, Pahle, Jürgen, Claydon, Amy J., Beynon, R. J. (Robert J.), Duncan, John, Westerhoff, Hans V., Mendes, Pedro and McCarthy, John E. G. (2013) An in vivo control map for the eukaryotic mRNA translation machinery. Molecular Systems Biology, Vol.9 . p. 635. doi:10.1038/msb.2012.73
|
HTML
WRAP_Firczuk_msb201273.pdf - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (1453Kb) | Preview |
Official URL: http://dx.doi.org/10.1038/msb.2012.73
Abstract
Rate control analysis defines the in vivo control map governing yeast protein synthesis and generates an extensively parameterized digital model of the translation pathway. Among other non-intuitive outcomes, translation demonstrates a high degree of functional modularity and comprises a non-stoichiometric combination of proteins manifesting functional convergence on a shared maximal translation rate. In exponentially growing cells, polypeptide elongation (eEF1A, eEF2, and eEF3) exerts the strongest control. The two other strong control points are recruitment of mRNA and tRNAi to the 40S ribosomal subunit (eIF4F and eIF2) and termination (eRF1; Dbp5). In contrast, factors that are found to promote mRNA scanning efficiency on a longer than-average 5′untranslated region (eIF1, eIF1A, Ded1, eIF2B, eIF3, and eIF5) exceed the levels required for maximal control. This is expected to allow the cell to minimize scanning transition times, particularly for longer 5′UTRs. The analysis reveals these and other collective adaptations of control shared across the factors, as well as features that reflect functional modularity and system robustness. Remarkably, gene duplication is implicated in the fine control of cellular protein synthesis.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Library of Congress Subject Headings (LCSH): | Messenger RNA, Yeast -- Physiology, Proteins -- Synthesis, Genetic translation | ||||
| Journal or Publication Title: | Molecular Systems Biology | ||||
| Publisher: | Nature Publishing Group | ||||
| ISSN: | 1744-4292 | ||||
| Official Date: | 2013 | ||||
| Dates: |
|
||||
| Volume: | Vol.9 | ||||
| Page Range: | p. 635 | ||||
| DOI: | 10.1038/msb.2012.73 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Open Access | ||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
