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Determination of types and binding sites of advanced glycation end products for substance P
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Lopez-Clavijo, Andrea F., Barrow, Mark P., Rabbani, Naila, Thornalley, Paul J. and O’Connor, Peter B. (2012) Determination of types and binding sites of advanced glycation end products for substance P. Analytical Chemistry, Vol.84 (No.24). pp. 10568-10575. doi:10.1021/ac301583d
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Official URL: http://dx.doi.org/10.1021/ac301583d
Abstract
Glycation by endogenous dicarbonyl metabolites such as glyoxal is an important spontaneous post-translational (PTM) modification of peptides and proteins associated with structural and functional impairment. The aim of this study was to investigate types and site of PTM of glyoxal-derived advanced glycation end-products–in the neuropeptide substance P by ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR), mass spectrometry, and tandem mass spectrometry (MS/MS) experiments. The main site of PTM by glyoxal was the side chain guanidine moiety of the arginine residue. Binding site identification has been achieved by electron capture dissociation, double-resonance electron capture dissociation, and collision-activated dissociation, with assignment of the modified amino acid residue with mass error <1 ppm.
| Item Type: | Journal Article | ||||
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | Analytical Chemistry | ||||
| Publisher: | American Chemical Society | ||||
| ISSN: | 0003-2700 | ||||
| Official Date: | 2012 | ||||
| Dates: |
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| Volume: | Vol.84 | ||||
| Number: | No.24 | ||||
| Page Range: | pp. 10568-10575 | ||||
| DOI: | 10.1021/ac301583d | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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