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The microrheology of sickle hemoglobin gels

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Zakharov, Mikhail N., Aprelev, Alexey, Turner, Matthew S. and Ferrone, F. A.. (2010) The microrheology of sickle hemoglobin gels. Biophysical Journal, Vol.99 (No.4). pp. 1149-1156. ISSN 0006-3495

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Official URL: http://dx.doi.org/10.1016/j.bpj.2010.04.079

Abstract

Sickle cell disease is a rheological disease, yet no quantitative rheological data exist on microscopic samples at physiological concentrations. We have developed a novel method for measuring the microrheology of sickle hemoglobin gels, based on magnetically driven compression of 5- to 8-mu m-thick emulsions containing hemoglobin droplets similar to 80 mu m in diameter. Using our method, by observing the expansion of the droplet area as the emulsion is compressed, we were able to resolve changes in thickness of a few nanometers with temporal resolution of milliseconds. Gels were formed at various initial concentrations and temperatures and with different internal domain structure. All behaved as Hookean springs with Young's modulus from 300 to 1500 kPa for gels with polymerized hemoglobin concentration from 6 g/dl to 12 g/dl. For uniform, multidomain gels, Young's modulus mainly depended on the terminal concentration of the gel rather than the conditions of formation. A simple model reproduced the quadratic dependence of the Young's modulus on the concentration of polymerized hemoglobin. Partially desaturated samples also displayed quadratic concentration dependence but with a smaller proportionality coefficient, as did samples that were desaturated in steps; such samples were significantly less rigid than gels formed all at once. The magnitude of the Young's modulus provides quantitative support for the dominant models of sickle pathophysiology.

Item Type:

Journal Article

Subjects:

Q Science > QH Natural history > QH301 Biology
R Medicine > RC Internal medicine

Divisions:

Faculty of Science > Physics

Library of Congress Subject Headings (LCSH):

Sickle cell anemia -- Pathophysiology, Rheology (Biology), Polymers -- Rheology, Hemoglobin

Journal or Publication Title:

Biophysical Journal

Publisher:

Biophysical Society

ISSN:

0006-3495

Official Date:

18 August 2010

Dates:

Date	Event
18 August 2010	Published

Volume:

Vol.99

Number:

No.4

Number of Pages:

Page Range:

pp. 1149-1156

Identification Number:

10.1016/j.bpj.2010.04.079

Status:

Peer Reviewed

Publication Status:

Published

Funder:

National Institutes of Health (U.S.) (NIH)

Grant number:

R01 HL057549 (NIH), P01HL058512 (NIH)

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