Zakharov, Mikhail N., Aprelev, Alexey, Turner, Matthew S. and Ferrone, F. A.. (2010) The microrheology of sickle hemoglobin gels. Biophysical Journal, Vol.99 (No.4). pp. 1149-1156. ISSN 0006-3495

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Abstract

Sickle cell disease is a rheological disease, yet no quantitative rheological data exist on microscopic samples at physiological concentrations. We have developed a novel method for measuring the microrheology of sickle hemoglobin gels, based on magnetically driven compression of 5- to 8-mu m-thick emulsions containing hemoglobin droplets similar to 80 mu m in diameter. Using our method, by observing the expansion of the droplet area as the emulsion is compressed, we were able to resolve changes in thickness of a few nanometers with temporal resolution of milliseconds. Gels were formed at various initial concentrations and temperatures and with different internal domain structure. All behaved as Hookean springs with Young's modulus from 300 to 1500 kPa for gels with polymerized hemoglobin concentration from 6 g/dl to 12 g/dl. For uniform, multidomain gels, Young's modulus mainly depended on the terminal concentration of the gel rather than the conditions of formation. A simple model reproduced the quadratic dependence of the Young's modulus on the concentration of polymerized hemoglobin. Partially desaturated samples also displayed quadratic concentration dependence but with a smaller proportionality coefficient, as did samples that were desaturated in steps; such samples were significantly less rigid than gels formed all at once. The magnitude of the Young's modulus provides quantitative support for the dominant models of sickle pathophysiology.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology R Medicine > RC Internal medicine
Divisions:	Faculty of Science > Physics
Library of Congress Subject Headings (LCSH):	Sickle cell anemia -- Pathophysiology, Rheology (Biology), Polymers -- Rheology, Hemoglobin
Journal or Publication Title:	Biophysical Journal
Publisher:	Biophysical Society
ISSN:	0006-3495
Date:	18 August 2010
Volume:	Vol.99
Number:	No.4
Number of Pages:	8
Page Range:	pp. 1149-1156
Identification Number:	10.1016/j.bpj.2010.04.079
Status:	Peer Reviewed
Publication Status:	Published
Funder:	National Institutes of Health (U.S.) (NIH)
Grant number:	R01 HL057549 (NIH), P01HL058512 (NIH)
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URI:	http://wrap.warwick.ac.uk/id/eprint/5344

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